TY  - EJOU
AU  - Huang, Xiaokui 
AU  - Xue, Yingbin 
AU  - Khan, Aaqil 
AU  - Hu, Hanqiao 
AU  - Feng, Naijie 
AU  - Zheng, Dianfeng 

TI  - Cloning and Functional Validation of Mung Bean <i>VrPR </i>Gene
T2  - Phyton-International Journal of Experimental Botany

PY  - 2023
VL  - 92
IS  - 8
SN  - 1851-5657

AB  - For the purpose of functional validation, the mung bean (<i>Vigna radiata</i>) <i>VrPR</i> gene was cloned and overexpressed
in <i>Arabidopsis thaliana</i>. The findings revealed that the ORF of <i>VrPR</i> contained 1200 bp, in which 399 amino acids
were encoded. Bioinformatics analysis showed that the <i>VrPR</i> protein belonged to the NADB Rossmann superfamily, which was one of the non-transmembrane hydrophilic proteins. <i>VrPR</i> was assumed to have 44 amino acid
phosphorylation sites and be contained in chloroplasts. The <i>VrPR</i> secondary structure comprised of random coil,
α helix, β angle, and extended chain, all of which were quite compatible with the anticipated tertiary structure.
Moreover, analysis of the phylogenetic tree indicated that the soybean PR (Glyma.12G222200) and <i>VrPR</i> were
closely related. Furthermore, chlorophyll content in leaves is markedly increased in Arabidopsis when <i>VrPR</i> is
overexpressed. Our findings will serve as a reference for more functional studies on the PR genes in mung bean.
KW  - Mung bean; gene cloning; <i>VrPR</i>; transgenic arabidopsis; functional verification

DO  - 10.32604/phyton.2023.027457