@Article{phyton.2023.029845,
AUTHOR = {Zijian Cao, Guoxia Xue, Lingfeng Hu, Haoxian Qu, Shuang Liang, Jisen Shi, Jinhui Chen, Zhaodong Hao},
TITLE = {Overexpression of the <i>LcPIN2</i> and <i>LtPIN2</i> Gene in <i>Arabidopsis thaliana</i> Promotes Root Elongation},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {92},
YEAR = {2023},
NUMBER = {8},
PAGES = {2383--2397},
URL = {http://www.techscience.com/phyton/v92n8/53338},
ISSN = {1851-5657},
ABSTRACT = {The auxin polar transporter, PIN-FORMED 2 (PIN2) plays an important role in root development. However, it remains unclear whether <i>PIN2</i> genes form two <i>Liriodendron</i> species, <i>L. chinense (<i>LcPIN2</i>)</i> and <i>L. tulipifera (<i>LtPIN2</i>)</i>, are both involved in root development and whether and to what extent these two genes diverge in function. Here, we cloned and overexpressed <i>LcPIN2</i> and <i>LtPIN2</i> in A<i>rabidopsis thaliana</i> wild-type (WT) and <i>Atpin2</i> mutant. Phylogenetic and sequence analysis showed a small degree of differentiation between these two <i>Liriodendron PIN2</i> genes. Tissue-specific gene expression analysis indicated that both <i>Liriodendron PIN2</i> genes were highly expressed in roots, implying a potential role in root development. Finally, heterologous overexpression of <i>LcPIN2</i> and <i>LtPIN2</i> in <i>Arabidopsis</i> both significantly increased the root length compared to wild-type and empty vector. Furthermore, the root length defect in <i>Atpin2</i> was complemented both by <i>LcPIN2</i> and <i>LtPIN2</i>. However, heterologous overexpression of <i>LcPIN2</i> and <i>LtPIN2</i> cannot rescue the defect in root gravitropism of <i>Atpin2</i> mutants. Taken together, our findings unravel <i>PIN2</i> genes from the magnoliids plant <i>Liriodendron</i> were functionally conserved with AtPIN2 in the dicotyledonous plant <i>Arabidopsis</i> in regard to the regulation of root length, but not root gravitropism. This study also provides a potential target for genetic improvement of the root system in these valuable forest trees <i>Liriodendron</i>.},
DOI = {10.32604/phyton.2023.029845}
}