@Article{phyton.2023.029784,
AUTHOR = {José Adrian Perez-Robles, Carlos Alberto Lecona-Guzmán, Víctor Manuel Ruíz-Valdiviezo, Joaquín Adolfo Montes-Molina},
TITLE = {Effects of Plant Extracts and <i>Beauveria bassiana</i> on the Activity of Defense-Related Enzymes in <i>Solanum lycopersicum</i> L. during Interaction with <i>Fusarium oxysporum</i> f. sp. <i>lycopersici</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {92},
YEAR = {2023},
NUMBER = {9},
PAGES = {2503--2518},
URL = {http://www.techscience.com/phyton/v92n9/53605},
ISSN = {1851-5657},
ABSTRACT = {The objective to this work was to evaluate the enzymatic activity in the culture of <i>Solanum lycopersicum</i> L. infected with <i>Fusarium oxysporum</i> after the combined application of <i>Beauveria bassiana</i> and plant extracts. <i>Solanum lycopersicum</i> plantlets were transplanted 15 days after the emergency. Five days after transplanting, <i>Beauveria bassiana</i> spores were applied at a concentration of 1 × 10<sup>7</sup> spores mL<sup>−1</sup> onto soil (along with <i>A. indica</i> (N) and <i>P. auritum</i> (H) leaf extracts) where <i>S. lycopersicum</i> plants were planted. Eight days after transplanting, spores of <i>F. oxysporum</i> strain were applied at a concentration of 1 × 10<sup>6</sup> spores mL<sup>−1</sup> to soil where <i>S. lycopersicum</i> plants were growing. The development of <i>S. lycopersicum</i> plants was monitored for 114 days, whereby a randomized complete block treatment design was used, the roots of the plants were crushed with liquid nitrogen, after which UV/VIS spectrophotometry was used to determine protein concentration, as well as the activity of <i>β</i>-1,3-glucanases, peroxidases (POX, EC1.11.1.7), catalase and chitinases. The treatments combining <i>B. bassiana</i> and <i>A. indica</i> and <i>P. auritum</i> extracts, had a significant difference in enzymatic activity levels, thus contributing to better defense mechanisms and a greater protection to <i>S. lycopersicum</i> plants in the presence of <i>F. oxysporum</i>. The application of <i>B. bassiana</i> and plant extracts to the ground mitigated damage caused by <i>F. oxysporum</i> on <i>S. lycopersicum</i> plants.},
DOI = {10.32604/phyton.2023.029784}
}