TY  - EJOU
AU  - Guo, Xiaolan 
AU  - Hu, Jinbin 
AU  - Yang, Shimei 
AU  - Wang, Delu 
AU  - Wang, Jianbing 

TI  - Genome-Wide Analysis of the <i>F3′5′H</i> Gene Family in Blueberry (<i>Vaccinium corymbosum</i> L.) Provides Insights into the Regulation of Anthocyanin Biosynthesis
T2  - Phyton-International Journal of Experimental Botany

PY  - 2023
VL  - 92
IS  - 9
SN  - 1851-5657

AB  - The F3′5′H gene family plays an important role in regulating anthocyanin biosynthesis, abiotic stress, and hormone signaling. In this study, 14 <i>F3′5′H</i> genes were identified from the blueberry genome. The chromosomal distribution, physicochemical properties, F3′5′H domain, conserved motifs, cis-acting elements, and intron/exon compositions were analyzed. The functional prediction analysis of these <i>VcF3′5′Hs</i> indicated that their biological functions included light response and other secondary metabolites. The results of qRT-PCR showed that <i>VcF3′5′Hs</i> (especially <i>VcF3′5′H4</i>) were highly expressed at the ripening stage. Subcellular localization revealed that <i>VcF3′5′H4</i> may be located in the endoplasmic reticulum. Co-expression analysis showed that the <i>VcF3′5′H</i> gene family was related to anthocyanin. This research provides an overview of the blueberry <i>F3′5′H</i> family and helps verify the role of these genes in regulating anthocyanin biosynthesis.
KW  - Blueberry; <i>F3′5′H</i>; anthocyanin; gene expression; co-expression

DO  - 10.32604/phyton.2023.029820