TY  - EJOU
AU  - Yang, Jing 
AU  - Pu, Tianxiunan 
AU  - Wan, Ke 
AU  - Wang, Linqi 
AU  - Shi, Yuanshuai 
AU  - Luo, Xu 
AU  - Tan, Jie 
AU  - Wang, Dongmei 
AU  - Liu, Yang 

TI  - Transcriptome and Metabolome Revealed the Mechanism of NtBRL3 Overexpression Tobacco (<i>Nicotiana tabacum</i> L. K326) in Response to Drought Stress
T2  - Phyton-International Journal of Experimental Botany

PY  - 2023
VL  - 92
IS  - 9
SN  - 1851-5657

AB  - Drought has severely affected the yield and quality of commercial crops. The <i>BRI1</i> family plays an important role in plant response to drought stress, and <i>BRL3</i> gene plays an important role in the study of drought in <i>Arabidopsis thaliana</i>. In this study, <i>NtBRL3</i> was constructed as a vector and genetically transformed to obtain ‘<i>N</i>. Tobacco K326’ overexpression of <i>NtBRL3</i>. The enzyme activities of transgenic tobacco and wild-type tobacco were measured and transcriptome and metabolome analyses were performed. The results showed that the antioxidant enzymes of transgenic tobacco were more active under drought conditions, and 85 significantly differentially metabolites and 106 significantly differentially expressed genes were identified in the metabolome and transcriptome analyses, respectively. Transgenic tobacco <i>NtBRL3ox</i> demonstrated an excessive accumulation of drought-related metabolites, sugars such as sucrose and maltotetraose, and amino acids such as proline, compared with WT. We discovered drought-related differential genes in the root transcriptome, among which <i>LOX6</i>, <i>RD22</i>, <i>WSD1</i>, <i>CCD8</i>, and <i>UGT</i> were key genes which play an important role in plant response to drought stress. Our results demonstrate that <i>NtBRL3</i> overexpression in K326 enhances drought resistance in transgenic tobacco.
KW  - <i>Nicotiana tabacum</i> L. K326; transcriptome; metabolome; <i>BRL3</i> gene; drought stress

DO  - 10.32604/phyton.2023.030301