@Article{phyton.2023.031228,
AUTHOR = {Mingda Yin, Rui Luo, Tana Liang, Qi Wen, Xiaotian Liang, Yanpeng Wen, Xuemei Hu, Zhiyan Wang, Chang Gao, Wenjing Ren, Fenglan Huang},
TITLE = {Effects of Heterologously Overexpressing <i>PIP5K</i>-Family Genes in Arabidopsis on Inflorescence Development},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {93},
YEAR = {2024},
NUMBER = {1},
PAGES = {97--117},
URL = {http://www.techscience.com/phyton/v93n1/55253},
ISSN = {1851-5657},
ABSTRACT = {Castor is one of the top 10 oil crops in the world and has extremely valuable uses. Castor inflorescences directly affect yield, so the study of inflorescence development is very important in increasing castor yield. Our previous studies have shown that the <i>PIP5K</i> gene family (<i>PIP5Ks</i>) is associated with inflorescence development. In this study, to determine the function of each <i>PIP5K</i> gene in castor, a female Lm-type castor line, aLmAB2, was used to determine the relative expression levels of the <i>PIP5Ks</i> in castor inflorescences. Six <i>PIP5K</i> genes were heterologously overexpressed in <i>Arabidopsis thaliana</i>, the relative expression of each gene and the effect on plants was determined in <i>A. thaliana</i>, and the relationships among the <i>PIP5Ks</i> in castor were inferred. The expression levels of the <i>PIP5Ks</i> in the female Lm-type castor line aLmAB2 were analyzed. The relative expression levels of the <i>PIP5K9</i> and <i>PIP5K11</i> genes were high (<i>p</i> < 0.05) in isofemale inflorescences, and those of <i>PIP5K1, PIP5K2, PIP5K6</i>, and <i>PIP5K8</i> were high (<i>p</i> < 0.05) in female inflorescences but low (<i>p</i> < 0.05) in bisexual inflorescences. The <i>PIP5Ks</i> were heterologously overexpressed in <i>A. thaliana</i>, and T3-generation plants with stable genetic resistance, i.e., AT-<i>PIP5K</i><sup>+</sup> plants (AT-<i>PIP5K1<sup>+</sup></i>, AT-<i>PIP5K2<sup>+</sup></i>, AT-<i>PIP5K6<sup>+</sup></i>, AT-<i>PIP5K8<sup>+</sup></i>, AT-<i>PIP5K9<sup>+</sup></i>, and AT-<i>PIP5K11<sup>+</sup></i> plants), were obtained. Biological tests of the AT-<i>PIP5K<sup>+</sup></i> plants showed that the growth of the main stem was significantly delayed in AT-<i>PIP5K<sup>+</sup></i> plants compared with Columbia wild-type (WT) <i>A. thaliana</i> plants; the <i>PIP5K1</i> and <i>PIP5K2</i> genes promoted lateral stem growth and flower and silique development; and the <i>PIP5K6</i>, <i>PIP5K8</i>, <i>PIP5K9</i> and <i>PIP5K11</i> genes inhibited lateral stem growth and flower and silique development. The correlations among PIP5Ks in castor suggest that there may be a synergistic relationship among <i>PIP5K1</i>, <i>PIP5K2</i>, and <i>PIP5K6</i> in castor inflorescences, and <i>PIP5K8</i>, <i>PIP5K9</i>, and <i>PIP5K11</i> are complementary to the other three genes.},
DOI = {10.32604/phyton.2023.031228}
}