@Article{phyton.2023.044996,
AUTHOR = {Yanhuan Lin, Hua Du, Zhitao Ye, Shuqi Wang, Zhen Wang, Xiaojuan Liu},
TITLE = {Protein Disulfide Isomerase and Its Potential Function on Endoplasmic Reticulum Quality Control in Diatom <i>Phaeodactylum tricornutum</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {93},
YEAR = {2024},
NUMBER = {1},
PAGES = {137--150},
URL = {http://www.techscience.com/phyton/v93n1/55254},
ISSN = {1851-5657},
ABSTRACT = {PDI is a molecular chaperone and plays an important role in Endoplasmic Reticulum quality control (ERQC). PDI participates in the refolding of the misfolded/unfolded proteins to maintain cellular homeostasis under different stresses. However, bioinformatic characteristics and potential functions of PDIs in diatom <i>Phaeodactylum tricornutum</i> (Pt) are still unknown so far. Hence, the genome-wide characteristics of PtPDI proteins in <i>P. tricornutum</i> were first studied via bioinformatic and transcriptomic methods. 42 PtPDI genes were identified from the genome of <i>P. tricornutum</i>. The motif, protein structure, classification, number of introns, phylogenetic relationship, and the expression level of 42 PtPDI genes under the tunicamycin stress were analyzed. A pair of tandem duplicated genes (PtPDI15 and PtPDI18) was observed in <i>P. tricornutum</i>. The 42 PtPDIs with different gene characteristics were divided into three independent clades, indicating different evolutional relationships and functions of these PtPDIs. The 14 up-regulated PtPDI genes under the tunicamycin treatment might have a positive effect on the ER quality control of the unfolded/misfolded proteins, while the 7 down-regulated PtPDIs might negatively affect the ERQC. The characteristics of all 42 PtPDIs and their proposed working model here provide a comprehensive understanding of the PtPDIs gene family. The differential expression of 21 PtPDIs will be useful for further functional study in the ERQC.},
DOI = {10.32604/phyton.2023.044996}
}