TY  - EJOU
AU  - Lin, Yanhuan 
AU  - Feng, Jiaxin 
AU  - Fang, Hao 
AU  - Huang, Wei 
AU  - Guo, Kanglie 
AU  - Liu, Xiyan 
AU  - Wang, Shuqi 
AU  - Liu, Xiaojuan 

TI  - The Expression Characteristics and Potential Functions of Heat Shock Factors in Diatom <i>Phaeodactylum tricornutum</i>
T2  - Phyton-International Journal of Experimental Botany

PY  - 2024
VL  - 93
IS  - 10
SN  - 1851-5657

AB  - Heat shock transcription factor (HSF) are essential regulators of heat shock protein (<i>HSP</i>) gene expression in plants and algae, contributing to their resilience against biotic and abiotic stresses. However, the localization, structure, phylogenetic relationship, and characteristics of <i>PtHSF</i> genes in microalgae, especially in diatom <i>Phaeodactylum tricornutum</i>, remain largely unexplored. This study presents a comprehensive analysis of the PtHSF gene family in <i>P. tricornutum</i>. A genome-wide analysis identified 68 PtHSF genes, which were classified into two distinct subfamilies: traditional and untraditional. Motif and structure analyses revealed evidence of multiple duplication events within the <i>PtHSF</i> gene family. Expression profiling revealed diurnal patterns, with 34 genes being downregulated during the light period and upregulated during the dark period, while 19 genes exhibited the opposite pattern. These findings suggest that <i>PtHSF</i> genes may have specialized functions during the diurnal cycle and play a crucial role in maintaining cellular homeostasis in response to various stresses. Notably, PtHSF16, 30, and 43 genes exhibited higher expression levels, suggesting their potential importance. This study provides a valuable foundation for future investigations into the specific functions of HSFs under different stress conditions and their regulatory mechanisms in <i>P. tricornutum</i> and other microalgae.
KW  - Diurnal cycle; heat shock factor; diatom; cellular homeostasis; differentially expressed gene

DO  - 10.32604/phyton.2024.055616