TY  - EJOU
AU  - Jiang, Yue 
AU  - Liu, Lingyu 
AU  - Wang, Kangyu 
AU  - Zhao, Mingzhu 
AU  - Chen, Ping 
AU  - Lei, Jun 
AU  - Wang, Yanfang 
AU  - Zhang, Meiping 
AU  - Wang, Yi 
AU  - Chen, Guang 

TI  - Response of the Ginseng C<sub>2</sub>H<sub>2</sub>-Type Zinc Finger Protein Family <i>PgZFPs</i> Gene to Methyl Jasmonate Regulation
T2  - Phyton-International Journal of Experimental Botany

PY  - 2024
VL  - 93
IS  - 11
SN  - 1851-5657

AB  - The main active components of ginseng are ginsenosides, which play significant roles in treating cardiovascular diseases, cancer, and providing antioxidant effects. Ginsenosides are primarily synthesized through the mevalonate pathway and the methylerythritol phosphate pathway. Many key enzyme genes involved in this biosynthetic process have been cloned and validated, yet the regulatory functions of transcription factors remain unclear. The C<sub>2</sub>H<sub>2</sub>-type zinc finger protein family, one of the largest families of transcription factors, is crucial in plant growth and development, response to biotic and abiotic stresses, and regulation of secondary metabolism. This study, based on the ginseng transcriptome database from Jilin, conducted a correlation analysis between the expression levels of <i>PgZFPs</i> genes in the Jilin ginseng C<sub>2</sub>H<sub>2</sub>-type zinc finger protein family and ginsenoside content, a genome-wide association study of <i>PgZFPs</i>, and co-expression analysis of <i>PgZFPs</i> with validated key enzyme genes. Ultimately, five candidate genes involved in ginsenoside biosynthesis were identified. The involvement of <i>PgZFP27</i> and <i>PgZFP-59-02</i> genes from the <i>PgZFPs</i> family in the biosynthesis of ginsenosides was validated through <i>in vitro</i> methyl jasmonate (MeJA) induction experiments. This result provides new genetic resources for the biosynthesis of ginsenosides.
KW  - Ginsenosides; C<sub>2</sub>H<sub>2</sub>-type zinc finger protein family; MeJA induction; functional study

DO  - 10.32604/phyton.2024.056384