@Article{phyton.2024.046989,
AUTHOR = {Shijie Lin, Zimo Wang, Hongbo Zhu, Conghui Wang, Hongfeng Wang, Dawei Zhang, Tianbing Gou, Guangdao Bao, Ye Luo, Huaijiang He, Zhonghui Zhang},
TITLE = {A New Micropropagation Technology of <i>Tilia amurensis</i>: <i>In Vitro</i> Micropropagation of Mature Zygotic Embryos and the Establishment of a Plant Regeneration System},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {93},
YEAR = {2024},
NUMBER = {2},
PAGES = {277--289},
URL = {http://www.techscience.com/phyton/v93n2/55519},
ISSN = {1851-5657},
ABSTRACT = {<i>Tilia amurensis</i> is an economically valuable broadleaf tree species in Northeast China. The production of high-quality <i>T. amurensis</i> varieties at commercial scales has been greatly limited by the low germination rates. There is thus a pressing need to develop an organogenesis protocol for <i>in vitro</i> propagation of <i>T. amurensis</i> to alleviate a shortage of high-quality <i>T. amurensis</i> seedlings. Here, we established a rapid <i>in vitro</i> propagation system for <i>T. amurensis</i> from mature zygotic embryos and analyzed the effects of plant growth regulators and culture media in different stages. We found that Woody plant medium (WPM) was the optimal primary culture medium for mature zygotic embryos. The highest callus induction percentage (68.76%) and number of axillary buds induced (3.2) were obtained in WPM + 0.89 µmol/L 6-benzyladenine (6-BA) + 0.46 µmol/L kinetin (KT) + 0.25 µmol/L indole-3-butryic acid (IBA) + 1.44 µmol/L gibberellin A3 (GA<sub>3</sub>). The multiple shoot bud development achieved the highest percentage (83.32%) in the Murashige and Skoog (MS) + 2.22 µmol/L 6-BA + 0.25 µmol/L IBA + 1.44 µmol/L GA<sub>3</sub>. The rooting percentage (96.70%) was highest in 1/2 MS medium + 1.48 µmol/L IBA. The survival percentage of transplanting plantlets was 82.22% in soil:vermiculite:perlite (5:3:1). Our study is the first to establish an effective organogenesis protocol for <i>T. amurensis</i> using mature zygotic embryos.},
DOI = {10.32604/phyton.2024.046989}
}