TY  - EJOU
AU  - Guo, Jialei 
AU  - Li, Fengzhen 
AU  - Shi, Guomin 
AU  - Zhao, Weimin 
AU  - He, Tao 

TI  - Analysis and Verification of the Conserved MYB Binding Element in the <i>DFR</i> Promoter in Compositae
T2  - Phyton-International Journal of Experimental Botany

PY  - 2024
VL  - 93
IS  - 2
SN  - 1851-5657

AB  - Anthocyanins, ubiquitous in the Compositae family, are regulated by MYB (v-myb avian myeloblastosis viral oncogene homolog), playing an important role in anthocyanin synthesis. In this study, we analyzed the regulation pathway in which the MYB protein of subgroup 6 promotes dihydroflavonol reductase (DFR) expression in Compositae, and validated this law in <i>Saussurea medusa</i> through yeast one-hybrid experiments. Our results showed that MYB and DFR underwent purification selection, <i>DFR</i> promoter analysis revealed the presence of MYB binding site (GAGTTGAATGG) and bHLH binding site (CANNTG) at the sense strand of 84–116 nucleotide residues from the start codon. These two motifs were separated by 9–10 nucleotide residues, as existed in the <i>DFR</i> promoters of many Compositae plants. Furthermore, the yeast one-hybrid experiment demonstrated that SmMYB1 can activate the promoter of <i>SmDFR</i>. Our results provide a reference for further functional study of <i>DFR</i> in Compositae.
KW  - Compositae; anthocyanin; <i>DFR</i> gene; <i>MYB</i> gene

DO  - 10.32604/phyton.2024.047429