@Article{phyton.2024.050321,
AUTHOR = {Haoxue Wu, Xiaohan Zhang, Xiaoyu Chen, Kang Li, Aixia Xu, Zhen Huang, Jungang Dong, Chengyu Yu},
TITLE = {Landscape of Sequence Variations in Homologous Copies of <i>FAD2</i> and <i>FAD3</i> in Rapeseed (<i>Brassica napus</i> L.) Germplasm with High/Low Linolenic Acid Trait},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {93},
YEAR = {2024},
NUMBER = {3},
PAGES = {627--640},
URL = {http://www.techscience.com/phyton/v93n3/56001},
ISSN = {1851-5657},
ABSTRACT = {Genetic manipulation (either restraint or enhancement) of the biosynthesis pathway of α-linolenic acid (ALA) in seed oil is an important goal in <i>Brassica napus</i> breeding. <i>B. napus</i> is a tetraploid plant whose genome often harbors four and six homologous copies, respectively, of the two fatty acid desaturases <i>FAD2</i> and <i>FAD3</i>, which control the last two steps of ALA biosynthesis during seed oil accumulation. In this study, we compared their promoters, coding sequences, and expression levels in three high-ALA inbred lines 2006L, R8Q10, and YH25005, a low-ALA line A28, a low-ALA/high-oleic-acid accession SW, and the wildtype ZS11. The expression levels of most <i>FAD2</i> and <i>FAD3</i> homologs in the three high-ALA accessions were higher than those in ZS11 and much higher than those in A28 and SW. The three high-ALA accessions shared similar sequences with the promoters and CDSs of <i>BnFAD3.C4</i> and <i>BnFAD3.A3</i>. In A28 and SW, substitution of three amino acid residues in BnFAD2.A5 and BnFAD2.C5, an absence of <i>BnFAD2.C1</i> locus, and a 549 bp long deletion on the <i>BnFAD3.A3</i> promoter were detected. The profile of <i>BnFAD2</i> mutation in the two low-ALA accessions A28 and SW is different from that reported in previous studies. The mutations in <i>BnFAD3</i> in the high-ALA accessions are reported for the first time. In identifying the sites of these mutations, we provide detailed information to aid the design of molecular markers for accelerated breeding schemes.},
DOI = {10.32604/phyton.2024.050321}
}