@Article{phyton.2024.049150,
AUTHOR = {Fenglan Wang, Zhenyuan Cai, Zhimei Li, Shilong Zhang, Honghui Luo, Qing Wu, Hanhan Xia, Yanhong Guo},
TITLE = {<i>FLOWERING LOCUS T (FT)</i> in Photosensitive Type <i>Chrysanthemum</i> Accelerates Flowering in <i>Arabidopsis</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {93},
YEAR = {2024},
NUMBER = {4},
PAGES = {819--830},
URL = {http://www.techscience.com/phyton/v93n4/56361},
ISSN = {1851-5657},
ABSTRACT = {<i>FLOWERING LOCUS T</i> (<i>FT</i>) is an important factor for integrating flowering signals through the photoperiod pathway, which significantly promotes flowering. Most chrysanthemum varieties are short-day plants, sensitive to the photoperiod, which limits their ornamental and annual production supply. In this study, we aim to analyze the nutrients and flowering-related genes of chrysanthemums with different photoperiod types and to clone and verify the function of the flowering-related gene <i>CmFT</i>. We found that the formation of floral buds requires the accumulation of starch while consuming soluble sugars and the expression patterns of flowering-related genes <i>GIGANTEA</i> (<i>GI</i>), <i>CONSTANS</i> (<i>CO</i>), and <i>FT</i> in <i>C.morifolium</i> ‘Zilian’ and <i>C. morifolium</i> ‘Zihongtuogui’ had a certain synchronization during floral buds differentiation according to our quantitative validation, and the expression levels of <i>CmGI</i>, <i>CmCO</i> and <i>CmFT</i> in <i>C. morifolium</i> ‘Zihongtuogui’ were higher than those in <i>C. morifolium</i>. ‘Zilian’ in the later stage of differentiation. <i>CmFT</i> was cloned from photosensitive chrysanthemums-<i>C. morifolium</i> ‘Zihongtuogui’ and polypeptide alignment and phylogenetic analysis showed that <i>CmFT</i> was clustered in FT-like subfamily. In further functional verification, we obtained two <i>Arabidopsis</i> transgenic lines. Our results showed that <i>CmFT</i> transgenic ft mutant lines can significantly accelerate flowering in <i>Arabidopsis</i>. Thus, we can initially confirm that <i>CmFT</i> plays an important role in promoting flowering, which may be the key reason for the photosensitivity of <i>C. morifolium</i> ‘Zihongtuogui’. Overall, the results of this study are of great importance in revealing the flowering mechanism of different photoperiod types of chrysanthemums.},
DOI = {10.32604/phyton.2024.049150}
}