@Article{phyton.2024.050066,
AUTHOR = {Wubin Dai, Xiuting Ju, Guomin Shi, Jialei Guo, Tao He},
TITLE = {<i>ShCTR1</i> Interacts with <i>ShRBOH1</i> to Positively Regulate Aerenchyma Formation in <i>Saussurea inversa</i> through ROS Mediation},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {93},
YEAR = {2024},
NUMBER = {5},
PAGES = {1023--1042},
URL = {http://www.techscience.com/phyton/v93n5/56747},
ISSN = {1851-5657},
ABSTRACT = {<i>Saussurea inversa</i> is one of the Tibetan medicine with developed aerenchyma. In this study, we investigated the function of the <i>ShCTR1</i> gene related to the formation of aerenchyma. The study of the occurrence and formation of aerenchyma in <i>S. inversa</i> has certain theoretical significance in revealing the special mechanism of alpine plants adapting to the environment. The results of yeast two-hybrid experiments showed that <i>S. inversa ShCTR1</i> interacted with the <i>ShRBOH1</i>, which is a key producer of reactive oxygen species (ROS), and it was hypothesized that <i>ShCTR1</i> regulation of aerenchyma formation in <i>S. inversa</i> mainly mediated by ROS. Aerenchyma induction and inhibition experiments were carried out on <i>S. inversa</i> seedlings, it was shown that ROS inducer (AT) and Ethene (ETH) induced the formation of aerenchyma in <i>S. inversa</i> well in roots, stems, and leaves, and Ethylene inhibitor (1-MCP) and ROS inhibitor DPI (diphenylene iodonium) inhibited the formation of aerenchyma of <i>S. inversa</i> in different degrees, which proved that ROS and ethylene (ET) were positively correlated with the formation of aerenchyma. Meanwhile, AT and ETH well induced the expression of the <i>ShCTR1</i> gene, and 1-MCP and DPI inhibited the expression of the <i>ShCTR1</i> gene to different degrees, which proved that ROS and ET were also positively correlated with the expression of the <i>ShCTR1</i> gene. <i>ShCTR1</i> was transferred into tobacco (<i>Nicotiana tabacum</i> L.), then repeat the above experiment. It was shown that positive rate and porosity were lowest and highest after DPI and AT treatments, respectively, it showed a positive correlation, which proved that the <i>ShCTR1</i> gene was positively regulating the formation of aerenchyma. Overexpression of the <i>ShCTR1</i> gene increased the content of ROS, consequently, the regulation of aerenchyma formation by the <i>ShCTR1</i> gene is mainly mediated by ROS. It suggested that the formation of aerenchyma in <i>S. inversa</i> was related to hypoxia in the alpine environment, and <i>ShCTR1</i> interacts with <i>ShRBOH1</i> to positively regulate the formation of aerenchyma mainly mediated by ROS.},
DOI = {10.32604/phyton.2024.050066}
}