@Article{phyton.2024.050220,
AUTHOR = {Yuan Tian, Lulu Zhi, Ping Li, Xiangyang Hu},
TITLE = {The NAC Transcription Factor <i>ANAC089</i> Modulates Seed Vigor through the ABI5-VTC2 Module in <i>Arabidopsis thaliana</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {93},
YEAR = {2024},
NUMBER = {6},
PAGES = {1101--1116},
URL = {http://www.techscience.com/phyton/v93n6/57197},
ISSN = {1851-5657},
ABSTRACT = {Seed viability is an essential feature for genetic resource conservation as well as sustainable crop production. Long-term storage induces seed viability deterioration or seed aging, accompanied by the accumulation of toxic reactive oxygen species (ROS) to suppress seed germination. Controlled deterioration treatment (CDT) is a general approach for mimicking seed aging. The transcription factor <i>ANAC089</i> was previously reported to modulate seed primary germination. In this study, we evaluated the ability of <i>ANAC089</i> to control seed viability during aging. Compared with that in the wild-type line, the mutation of <i>ANAC089</i> significantly increased HO, thereby reducing seed viability after CDT, while the overexpression of <i>ANAC089</i> reduced HO and improved seed longevity, indicating a critical role for <i>ANAC089</i> in maintaining seed viability through HO signaling. A series of studies have shown that <i>ANAC089</i> targets and negatively regulates the level of <i>ABI5</i>, an important transmitter of abscisic acid (ABA) signals, to affect seed viability after CDT. Furthermore, <i>ABI5</i> negatively regulated the expression of <i>VTC2</i>, which is involved in the biosynthesis of the antioxidant ascorbic acid and HO scavenging. As a result, <i>ANAC089</i> attenuates the generation of HO, thereby enhancing seed viability through the ABI5-VTC2 module during the seed aging process. Taken together, our results reveal a novel mechanism by which ANAC089 enhances seed viability by coordinating <i>ABI5</i> and <i>VTC2</i> expression, ultimately preventing the overaccumulation of HO, which would have led to reduced seed viability.},
DOI = {10.32604/phyton.2024.050220}
}