@Article{phyton.2024.051517,
AUTHOR = {Junhui Yan, Yawen Wang, Qiong Li, Yu Zhou, Xu Wang, Li Luo},
TITLE = {The MtRGF6 Peptide Differentially Regulates Root Development and Symbiotic Nodulation of <i>Medicago truncatula</i> and <i>Lotus japonicus</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {93},
YEAR = {2024},
NUMBER = {6},
PAGES = {1237--1248},
URL = {http://www.techscience.com/phyton/v93n6/57205},
ISSN = {1851-5657},
ABSTRACT = {Rhizobia induces nitrogen-fixing nodules in legumes used in agricultural production, providing a direct source of combined nitrogen to leguminous crops. Small peptides, such as CLAVATA3/EMBRYO SURROUNDING REGION peptides (CLE), are known to regulate the formation and development of nitrogen-fixing nodules in legumes. Root meristem growth factor (RGF) peptides from <i>Medicago truncatula</i> not only regulate root development but also modulate nodulation symbiosis with <i>Sinorhizobium meliloti</i>. However, the impact of RGF peptides from one leguminous species on the others remains unclear. In this study, we investigate the effects of the RGF family peptide MtRGF6p from <i>M. truncatula</i> on nodulation symbiosis and root development in <i>Lotus japonicus</i>. The MtRGF6 gene is predominantly expressed in the root nodules of <i>M. truncatula</i> and shows low identity with RGF homologous genes from <i>L. japonicus</i>. The gene promoter is active in the primordia of root nodules and lateral roots, as well as in young nodules and roots, and the meristem, infection, and nitrogen-fixing regions of the mature nodule. Chemically synthesized MtRGF6p promoted primary root growth in <i>M. truncatula</i> but suppressed the growth of <i>L. japonicus</i> primary roots. The peptide negatively affected the initiation of nodule primordia, the formation of infection threads, and nodulation in both legumes, with a low dosage showing effects on <i>L. japonicus</i> compared to <i>M. truncatula</i>. These results suggest that the MtRGF6 peptide from <i>M. truncatula</i> may serve as an inter-species signal affecting the root organ development of <i>L. japonicus</i>.},
DOI = {10.32604/phyton.2024.051517}
}