@Article{phyton.2024.051652,
AUTHOR = {Dongbo Wu, Shuang Qu, Lianwen Shen, Shengqun Chen, Xia Jiang, Ai Rao, Yuxue Zhao, Yueyun Wang},
TITLE = {Metabolomic Analysis of the Anthocyanins Associated with Different Colors of <i>Cymbidium goeringii</i> in Guizhou, China},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {93},
YEAR = {2024},
NUMBER = {7},
PAGES = {1455--1466},
URL = {http://www.techscience.com/phyton/v93n7/57475},
ISSN = {1851-5657},
ABSTRACT = {<i>Cymbidium goeringii</i> is an economically important ornamental plant, and flower color is one of the main features of <i>C. goeringii</i> that contributes to its high economic value. To clarify the molecular mechanisms underlying the role of anthocyanins in mediating differences in color among varieties, liquid chromatography–tandem mass spectrometry was used to perform anthocyanin-targeted metabolomics of seven <i>C. goeringii</i> varieties, including ‘Jin Qian Yuan’ (JQY), ‘Jin Xiu Qian Yuan’ (JXQY), ‘Miao Jiang Su Die’ (MJSD), ‘Qian Ming Su’ (QMS), ‘Shi Chan’ (SC), and ‘Yang Ming Su’ (YMS), as well as the <i>C. goeringii</i>. We detected 64 anthocyanins, including cyanidins, delphinidins, malvidins, pelargonidins, peonidins, petunidins, procyanidins, and flavonoids. We identified six shared differentially accumulated metabolites (DAMs), including cyanidin-3-<i>O</i>-rutinoside, delphinidin-3-<i>O</i>-sophoroside, pelargonidin-3-<i>O</i>-rutinoside, peonidin-3-<i>O</i>-(6-<i>O</i>-malonyl-beta-D-glucoside), peonidin-3-<i>O</i>-sophoroside, and chalcone. Most DAMs were enriched in the anthocyanin biosynthesis pathway. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that the differentially expressed metabolites were significantly enriched in the anthocyanin biosynthesis pathway. Analysis of the content of differentially expressed metabolites indicated that peonidin-3-<i>O</i>-(6-<i>O</i>-malonyl-beta-D-glucoside) was the key metabolite underlying color differences among <i>C. goeringii</i> varieties. Procyanidin B2, pelargonidin-3-O-galactoside, and naringenin might also affect the color formation of JQY and QMS, SC, and MJSD, respectively. The results of this study shed light on the metabolic mechanism underlying flower color differences in <i>C. goeringii</i> at the molecular level. Our findings will aid future studies of the mechanism of flower color regulation in <i>C. goeringii</i> and have implications for the breeding of new varieties.},
DOI = {10.32604/phyton.2024.051652}
}