TY  - EJOU
AU  - Su, Dongxue 
AU  - Zheng, Jiarui 
AU  - Yi, Yuwei 
AU  - Zhang, Shuyuan 
AU  - Feng, Luxin 
AU  - Quzhen, Danzeng 
AU  - Qiong, De 
AU  - Zhang, Weiwei 
AU  - Wang, Qijian 
AU  - Xu, Feng 

TI  - Genome-Wide Identification of the <i>MYB</i> Gene Family and Screening of Potential Genes Involved in Fatty Acid Biosynthesis in Walnut
T2  - Phyton-International Journal of Experimental Botany

PY  - 2024
VL  - 93
IS  - 9
SN  - 1851-5657

AB  - The multifaceted roles of <i>MYB</i> transcriptional regulators are pivotal in orchestrating the complex processes of secondary metabolism, stress tolerance mechanisms, and life cycle progression and development. This study extensively examined the <i>JrMYB</i> genes using whole genome and transcriptomic data, focusing on identifying putative <i>MYB</i> genes associated with fatty acid metabolism. 126 <i>MYB</i> genes were identified within the walnut genome, characterized by hydrophilic proteins spanning lengths ranging from 78 to 1890 base pairs. Analysis of cis-acting elements within the promoter regions of <i>MYB</i> genes revealed many elements linked to cell development, environmental stress, and phytohormones. Transcriptomic data was utilized to examine the role of <i>JrMYB</i> genes in the biosynthesis of fatty acids in walnuts. The results revealed diverse expression of these genes across various tissue sites, displaying varying levels and distinct expression patterns. Furthermore, by integrating the results of the phylogenetic tree with the correlation of expression levels, a total of 10 genes potentially involved in the regulation of fatty acid synthesis were screened. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was conducted on these 10 genes and further identified 4 candidate genes, and a transcription regulatory network involved in fatty acids metabolism was constructed. This study presents a systematic analysis of <i>JrMYB</i> genes, laying the groundwork for an in-depth exploration of the <i>JrMYB</i> genes family’s function in regulating fatty acid synthesis.
KW  - Walnut; <i>JrMYB</i> genes; fatty acid synthesis; gene expression; bioinformatics analysis

DO  - 10.32604/phyton.2024.055350