@Article{phyton.2025.059387,
AUTHOR = {Fayez Althobaiti, Hadeer Darwish, Raghad Alruqayb, Saqer S. Alotaibi, Fahad E. Alharthi, Ibrahim Jafri, Sarah Awwadh Altalhi, Ghadi Alsharif, Hussam Awwadh E Althagafi, Hesham Noureldeen, Sarah Alharthi, Jawaher Albaqami, Najla Amin T. Al Kashgry, Ahmed Noureldeen},
TITLE = {Analyzing the Cytotoxic and Genetic Impact of <i>Datura stramonium</i> Extract on MCF7 and HT29 Cancer Cells: A Metabolite and Gene Expression Study},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {94},
YEAR = {2025},
NUMBER = {1},
PAGES = {181--198},
URL = {http://www.techscience.com/phyton/v94n1/59342},
ISSN = {1851-5657},
ABSTRACT = {
The interest in using the <i>Datura stramonium</i> plant is due to its natural products, which are used in many pharmaceutical industries. The objective of the current study was to assess the therapeutic and cytotoxic effects of the <i>D. stramonium</i> plant on two types of human cancer cell models (MCF7 and HT29) <i>in vitro</i>. A soxhlet apparatus was used to obtain methanolic extract from dried plant leaves. The recovered crude, after the solvent had evaporated, was then dispersed at varied concentrations of extract 100, 50, 20, and 0.0 µg/mL and tested to see how the cells responded. Also, the cancer-testis antigen (CTA) gene transcription in the two cell types exposed to the plant extract was examined using a semi-quantitative real-time polymerase chain reaction. Gas chromatography–mass spectrometry (GC-MS) results produced the significant main metabolites Nonanoic acid, Tropine N-Oxide, 3,6-Ditigloyloxy-7-hydroxytropane, Hexadecanoic acid, 2-Pentadecanone,6,10,14-trimethyl-, Carvenone, methyl ester, Phytol, Aposcopolamine, Hyoscyamine, 4,8,12,16-Tetramethylheptadecan-4-olide, Scopolamine, Alpha.-Tocospiro A, 1,2-Cycloheptanedione, 3,3,7,7-tetramethyl-, dihydrazone, Campesterol, Stigmasterol, Gamma -Sitosterol and dl-.alpha.-Tocopherol. The results showed that the two types of cell lines impacted by <i>D. stramonium</i> extract, through untreated type 1 cells (MCF7) gave a highly significant transcription according to all applicable genes. All implemented analyses cleared the strong genetic impacts of <i>Datura</i> extract on cancer cells’ genomes. <i>TGIF2LY</i> and <i>C2orf63</i> transcript accumulation were also significantly elevated when exposed to plant extract at a level of 50 µg/mL in cell line type 2 (HT29), but <i>TGIF2LY</i> and <i>P53</i> had the lowest relative expression at a level of 100 µg/mL when treated the same cell line type.},
DOI = {10.32604/phyton.2025.059387}
}