TY  - EJOU
AU  - Zhong, Mingxin 
AU  - Zhang, Yuanyuan 
AU  - Guo, Xinlei 
AU  - Zhang, Bainian 
AU  - Tan, Chengjia 
AU  - Xu, Zhuo 
AU  - Hu, Xin 
AU  - Feng, Daren 
AU  - Xi, Zhenpeng 
AU  - Wang, Qian 
AU  - Tian, Hui 

TI  - Identification and Expression Analysis of <i>AP2/ERF</i> Gene Family Members in Different Growth Periods of <i>Magnolia officinalis</i>
T2  - Phyton-International Journal of Experimental Botany

PY  - 2025
VL  - 94
IS  - 10
SN  - 1851-5657

AB  - <i>Magnolia officinalis</i> is a perennial deciduous tree that has medicinal properties. The <i>AP2/ERF</i> gene family has a number of roles in long-term growth and metabolism. The expression of this function varies with the growth period. In this work, based on the transcriptome data of <i>Magnolia officinalis</i>, the complete coding gene of <i>Magnolia officinalis</i> was obtained, and the corresponding protein sequence was retrieved from NCBI and compared with the model plant <i>Arabidopsis thaliana</i>. After screening, 75 protein sequences from the <i>AP2/ERF</i> gene family were identified and called <i>MoAP2/ERF1</i>–<i>MoAP2/ERF75</i>, followed by bioinformatics analysis. 75 <i>AP2/ERF</i> gene families were found and classified into four subfamilies. Their protein architectures had one or more conserved AP2 domains, which were typically unstable and hydrophilic. Subcellular research revealed that it was primarily located in the nucleus. Among them, the <i>DREB</i> subfamily showed stronger activity in the early growth period of <i>Magnolia officinalis</i>, suggesting that <i>Magnolia officinalis</i> had stronger resistance to adversity during this period. The 15 members of the <i>MoAP2/ERF</i> gene family showed significant differences during different growth periods, and they regulated the gene expression of <i>Magnolia officinalis</i> by binding to DNA. The 15 <i>MoAP2/ERF</i> gene families have a wide range of physiological activities in biological processes, cellular components, and molecular functions. Including <i>MoAP2/ERF55</i> can catalyze imidazole glycerol phosphate synthase activity; <i>MoAP2/ERF39</i> acts as a transcriptional activator of <i>Pti6</i>.
KW  - <i>Magnolia officinalis</i>; <i>AP2/ERF</i> gene family; bioinformatics; <i>DREB</i>; growing period

DO  - 10.32604/phyton.2025.070560