@Article{phyton.2025.071543,
AUTHOR = {Surelys Ramos-Bell, Edson Rayón-Díaz, Juan A. Herrera-González, Estefanía Martínez-Batista, Rita M. Velázquez-Estrada, Porfirio Gutiérrez–Martínez},
TITLE = {Ultrasound-Assisted Hydrothermal Treatment in Combination with Chitosan for Fungal Control of <i>Botrytis cinerea</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {94},
YEAR = {2025},
NUMBER = {10},
PAGES = {3145--3156},
URL = {http://www.techscience.com/phyton/v94n10/64295},
ISSN = {1851-5657},
ABSTRACT = {A significant portion of losses in the fruit and vegetable sector are caused by the necrotrophic fungus <i>Botrytis cinerea</i>, due to its high prevalence in over 1000 crop species. Identifying a technology capable of exclusively inhibiting its growth and development remains challenging; therefore, various treatments have been proposed to act synergistically in preventing the spread of this pathogen. The objective of this study was to evaluate an ultrasound-assisted treatment combined with chitosan to control the development of <i>B. cinerea</i>. <i>In vitro</i> analyses showed that combining temperature, ultrasound, and chitosan inhibited the fungal radial growth by 80%, reduced sporulation, and decreased germination. Electrolyte leak analysis indicated damage to the <i>B. cinerea</i> cell wall, causing intracellular material to escape into the environment. Scanning and transmission electron microscopy images also revealed severe structural damage, including loss of the cytoplasmic membrane and cell lysis.},
DOI = {10.32604/phyton.2025.071543}
}