TY - EJOU AU - Wu, Shaoyan AU - Tian, Jie AU - Wang, Yiyan AU - Arif, Muhammad AU - Wang, Shuyao AU - Wang, Jing AU - Yang, Zhuoyao AU - Xu, Ruhong AU - Li, Luhua TI - Genetic Mapping of Grain Length-and Width-Related Genes in the Local Wheat Variety Guizi 1×Zhongyan 96-3 Hybrid Population Using Genome Sequencing T2 - Phyton-International Journal of Experimental Botany PY - 2025 VL - 94 IS - 12 SN - 1851-5657 AB - Wheat grain morphology, particularly grain length (GL) and width (GW), is a key determinant of yield. To improve the suboptimal grain dimensions of the local anthocyanin-rich variety Guizi 1 (GZ1), we crossed it with Zhongyan 96-3 (ZY96-3), an elite germplasm known for faster grain filling and superior grain size. A genotyping-by-sequencing (GBS) approach was applied to an F2 population of 110 individuals derived from GZ1 × ZY96-3, resulting in the identification of 23,134 high-quality SNPs. Most of the SNPs associated with GL and GW were clustered on chromosomes 2B, 3A, and 3B. QTL mapping for GL revealed two major loci, GL1 on chromosome 2B and GL2 on chromosome 3B, and eight candidate genes were identified within their corresponding intervals (2B: 63.6–70.4 Mb; 3B: 631.5–633.3 Mb). These genes encode proteins potentially involved in grain size regulation, including a TOR2 regulation-associated protein, erect spike 2 (EP2), fibroblast growth factor 6 (FGF6), cellulose synthase-like (CSLD), RelA/pot homologue three family protein, and three GDSL esterase/lipase (GLIP) proteins. Additionally, we detected a QTL associated with GW on chromosome 3A and identified two candidate genes, TOR2 regulation and starch synthase within the 61.4–68.5 Mb interval. Overall, this study provides a strong theoretical and technical basis for wheat genetic improvement and offers valuable resources for precise QTL mapping and candidate gene discovery. KW - Wheat; grain length; grain width; genotyping-by-sequencing (GBS); QTL mapping; SNP markers; candidate genes; Guizi 1; Zhongyan 96-3 DO - 10.32604/phyton.2025.072229