TY  - EJOU
AU  - Wang, Guolan 
AU  - Xie, Ting 
AU  - Fu, Lijun 
AU  - Qu, Siying 
AU  - Li, Jie 

TI  - Research on the <i>Cymbidium tortisepalum</i> var. <i>longibracteatum</i> Growth and Non-Tube Rapid Propagation Based on Response Surface Methodology
T2  - Phyton-International Journal of Experimental Botany

PY  - 2025
VL  - 94
IS  - 3
SN  - 1851-5657

AB  - The objective of this study was to determine the optimal proportions of plant growth regulators for growth and non-tube rapid propagation of <i>Cymbidium tortisepalum</i> var. <i>longibracteatum</i>; seedlings were utilized as the material. The effects of various combinations and concentrations of 6-benzylaminopurine (6-BA), gibberellic acid (GA<sub>3</sub>), and naphthaleneacetic acid (NAA) on growth and non-tube rapid propagation were assessed through a single-factor testing and response surface methodology. The results indicated that 6-BA at 60 mg/L, GA<sub>3</sub> at 150 mg/L, and NAA at 30 mg/L were the most effective concentrations for promoting leaf buds formation in the single-factor analysis. Response surface methodology clarified the sensitivity of the proliferation rate of lateral buds to the three factors, with 6-BA being the most influential, followed by GA<sub>3</sub> and NAA. The increase in leaf area was most significantly influenced by NAA, then GA<sub>3</sub>, and least by 6-BA, while the increase in plant height was most responsive to GA<sub>3</sub>, followed by 6-BA, and then NAA. The ideal concentrations of plant growth regulators were established as 6-BA at 43 mg/L, GA<sub>3</sub> at 169 mg/L, and NAA at 36 mg/L. Under these conditions, the lateral bud number per plant was 2.78, with a leaf area increment of 2.87 cm<sup>2</sup> and a plant height increment of 2.67 cm.
KW  - <i>Cymbidium tortisepalum</i> var. <i>longibracteatum</i>; growth; non-tube rapid propagation; response surface methodology; plant growth regulator

DO  - 10.32604/phyton.2025.060634