TY  - EJOU
AU  - Qi, Weiliang 
AU  - Sun, Wancang 
AU  - Ma, Li 
AU  - Li, Xiaolong 
AU  - Liu, Haiqing 
AU  - Yang, Cairong 
AU  - Wei, Ziyao 

TI  - Pathways Related to ROS Production, Clearance, and Signal Transduction during Cold Response in <i>Brassica napus</i> L. with Strong Cold Resistance
T2  - Phyton-International Journal of Experimental Botany

PY  - 2025
VL  - 94
IS  - 3
SN  - 1851-5657

AB  - <i>Brassica napus</i> L. (<i>B. napus</i>), recognized as a significant cash and oil crop, faces challenges in popularization and application in northern China due to its limited cold resistance. Clarifying the mechanism of cold stress on gene regulation and signal transduction in <i>B. napus</i> is crucial. To address these issues, we conducted transcriptome sequencing and gene expression analysis, along with gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway profiling under natural (25°C) and cold (4°C) conditions in cold tolerant 16VHNTS309 and weak cold-resistant Tianyou 2238 <i>B. napus</i> seedlings. Enhanced genomic annotation was achieved through additional sequencing. A total of 6127 and 8531 differentially expressed genes (DEG) were identified in 16VHNTS309 and Tianyou 2238, respectively. The expression patterns of 23 DEGs were validated by quantitative real-time PCR (qRT-PCR), confirming the RNA-Seq results. Under cold stress, 58 pathways in 16VHNTS309 demonstrated significant changes (q-Value < 0.05), compared to 9 pathways in Tianyou 2238 (q-Value < 0.05), highlighting <i>B. napus’</i> sophisticated regulatory network which aids in managing growth and development challenges. After 48 h of cold stress treatment, genes associated with reactive oxygen species (ROS) clearance, such as those involved in antioxidant VB<sub>6</sub>, sulfur metabolism, peroxisomes, and phagosomes, were notably up-regulated in 16VHNTS309, indicating its robust ROS clearance capability. Significant gene expressions within Ca<sup>2+</sup>, MAPK, and transcription factor pathways related to ROS suggest that varieties with strong cold resistance possess a complex signal regulation mechanism. Comprehensive analyses of stomatal cells, physiological parameters of ROS, ABA, and H<sub>2</sub>S, along with transcriptomic data, revealed that optimal ROS levels interact with ABA and H<sub>2</sub>S to regulate stomatal closure in <i>B. napus</i> 16VHNTS309 under the influence of antioxidant enzymes.
KW  - <i>Brassica napus</i> L.; cold stress; comparative transcriptome; ROS

DO  - 10.32604/phyton.2025.060712