@Article{phyton.2025.061550,
AUTHOR = {Ran Li, Liuhong Chen, Yu Liu, Chunlei Zhao, Yanli Li, Guangzhou Ding},
TITLE = {Genomic Characterization of BvMLO Genes in Sugar Beet Focusing on BvMLO2 BvMLO7 Responses to <i>Cercospora beticola</i> and Abiotic Stress},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {94},
YEAR = {2025},
NUMBER = {3},
PAGES = {875--895},
URL = {http://www.techscience.com/phyton/v94n3/60202},
ISSN = {1851-5657},
ABSTRACT = {Mildew resistance locus O (MLO) proteins are extensively found in various plant species and are essential for multiple biological functions. The characterization and analysis of <i>MLO</i> genes have been conducted across numerous species. However, the functions and features of <i>MLO</i> genes inside sugar beet remain poorly understood. In the present research, we conducted a comprehensive analysis of the structural features of <i>MLO</i> genes, physicochemical characteristics of proteins, evolutionary connections, and expression profiles in sugar beet. A total of 13 <i>BvMLO</i> genes containing <i>MLO</i> structural domains were detected and renamed based on their locations on chromosomes within the sugar beet genome. According to the classification of <i>AtMLO</i> genes, the evolutionary analysis revealed that these 13 <i>BvMLO</i> genes were classified into three subgroups and unevenly located across four chromosomes. Synteny and collinearity analysis confirmed that gene clusters occurred during the evolution of the <i>BvMLO</i> gene family. Examination of cis-regulatory elements revealed specific stress-induced and hormone-associated components within the regulatory regions of <i>BvMLOs</i>. We also found that the expression levels of <i>BvMLO2</i> and <i>BvMLO7</i> cloned from sugar beet plants inoculated by <i>Erysiphe betae</i> (Vanha) were significantly regulated by <i>Cercospora beticola Sacc</i> (<i>C. beticola</i>), which indicated that they might both participate in some disease resistance processes. Moreover, quantitative real-time PCR (qRT-PCR) results confirmed that <i>BvMLO2</i> and <i>BvMLO7</i> were involved in plant resistance to various biotic and abiotic stress factors. Overall, this research provides a fundamental basis for upcoming studies on the functions and control mechanisms of <i>BvMLO</i> genes within sugar beet. These research findings help advance the progress of disease-resistant breeding in sugar beet and enhance the effectiveness of its resistance breeding.},
DOI = {10.32604/phyton.2025.061550}
}