@Article{phyton.2025.063896,
AUTHOR = {Bouslamti Mohammed, Nouioura Ghizlane, Tbatou Widad, Mohamed Chebaibi, Abdulsalam Alhalmi, Najoua Soulo, Sulaiman Mohammed Alnasser, Fahad M Alshabrmi, Amine Elbouzidi, Lyoussi Badiaa, Benjelloun Ahmed Samir},
TITLE = {HPLC-DAD Profiling and Diuretic Effect of <i>Solanum elaeagnifolium</i> (Cav.) Aqueous Extract: A Combined Experimental and Computational Approach},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {94},
YEAR = {2025},
NUMBER = {5},
PAGES = {1505--1518},
URL = {http://www.techscience.com/phyton/v94n5/61373},
ISSN = {1851-5657},
ABSTRACT = {The <i>Solanum</i> genus is known for its diverse bioactive compounds, yet its diuretic potential remains understudied. This research commenced with an analysis of polyphenol and flavonoid content in <i>Solanum elaeagnifolium</i> leaf extract (SEFE) using colorimetric techniques, followed by HPLC-DAD to delineate its chemical composition. The aqueous extract revealed prominent constituents: naringin (12.38%), quercetin 3-O-B-D-Glucoside (27.25%), and flavone (15.26%). A 15-day study on normal rats investigated the diuretic potential of SEFE at repeated doses. SEFE significantly increased urine volume and urinary sodium/potassium levels without inducing hypokalaemia, contrasting with furosemide, a standard diuretic that induced hypokalaemia. Conversely, furosemide, a standard diuretic, increased urinary sodium and potassium while inducing hypokalaemia. It was evident that the diuretic effect of <i>S. elaeagnifolium is</i> dose-dependent, with a dosage of 500 mg/kg body weight exerting a more potent diuretic effect compared to furosemide. The diuretic activity of this plant was supported by an <i>in silico</i> study of the diuretic effect. The findings demonstrate how <i>S. elaeagnifolium</i> leaves have a potent diuretic impact on rats. However, more in-depth studies are needed to examine the following aspects: identifying the specific molecules responsible for the diuretic effect, understanding the molecular and cellular mechanisms underlying this activity, and assessing the long-term safety and clinical efficacy of this plant in different contexts.},
DOI = {10.32604/phyton.2025.063896}
}