TY  - EJOU
AU  - Mohammed, Bouslamti 
AU  - Ghizlane, Nouioura 
AU  - Widad, Tbatou 
AU  - Chebaibi, Mohamed 
AU  - Alhalmi, Abdulsalam 
AU  - Soulo, Najoua 
AU  - Alnasser, Sulaiman Mohammed 
AU  - Alshabrmi, Fahad M 
AU  - Elbouzidi, Amine 
AU  - Badiaa, Lyoussi 
AU  - Samir, Benjelloun Ahmed 

TI  - HPLC-DAD Profiling and Diuretic Effect of <i>Solanum elaeagnifolium</i> (Cav.) Aqueous Extract: A Combined Experimental and Computational Approach
T2  - Phyton-International Journal of Experimental Botany

PY  - 2025
VL  - 94
IS  - 5
SN  - 1851-5657

AB  - The <i>Solanum</i> genus is known for its diverse bioactive compounds, yet its diuretic potential remains understudied. This research commenced with an analysis of polyphenol and flavonoid content in <i>Solanum elaeagnifolium</i> leaf extract (SEFE) using colorimetric techniques, followed by HPLC-DAD to delineate its chemical composition. The aqueous extract revealed prominent constituents: naringin (12.38%), quercetin 3-O-B-D-Glucoside (27.25%), and flavone (15.26%). A 15-day study on normal rats investigated the diuretic potential of SEFE at repeated doses. SEFE significantly increased urine volume and urinary sodium/potassium levels without inducing hypokalaemia, contrasting with furosemide, a standard diuretic that induced hypokalaemia. Conversely, furosemide, a standard diuretic, increased urinary sodium and potassium while inducing hypokalaemia. It was evident that the diuretic effect of <i>S. elaeagnifolium is</i> dose-dependent, with a dosage of 500 mg/kg body weight exerting a more potent diuretic effect compared to furosemide. The diuretic activity of this plant was supported by an <i>in silico</i> study of the diuretic effect. The findings demonstrate how <i>S. elaeagnifolium</i> leaves have a potent diuretic impact on rats. However, more in-depth studies are needed to examine the following aspects: identifying the specific molecules responsible for the diuretic effect, understanding the molecular and cellular mechanisms underlying this activity, and assessing the long-term safety and clinical efficacy of this plant in different contexts.
KW  - <i>S. elaeagnifolium</i>; urine volume; potassium; diuretic; furosemide; leaves; docking

DO  - 10.32604/phyton.2025.063896