@Article{phyton.2025.064068,
AUTHOR = {Lucas Leal Lima, Angélica Souza Gouveia, Analice Martins Duarte, Filipe Schitini Salgado, Nathália Silva Oliveira, Monique da Silva Bonjour, Iana Pedro da Silva Quadros, Maria Goreti Almeida Oliveira, Flavia Maria Silva Carmo, Elizabeth Pacheco Batista Fontes, Humberto Josué de Oliveira Ramos},
TITLE = {Differential Gene Expression and Metabolic Changes in Soybean Leaves Triggered by Caterpillar Chewing Sound Signals},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {94},
YEAR = {2025},
NUMBER = {6},
PAGES = {1787--1810},
URL = {http://www.techscience.com/phyton/v94n6/62652},
ISSN = {1851-5657},
ABSTRACT = {Sound contains mechanical signals that can promote physiological and biochemical changes in plants. Insects produce different sounds in the environment, which may be relevant to plant behavior. Thus, we evaluated whether signaling cascades are regulated differently by ecological sounds and whether they trigger molecular responses following those produced by herbivorous insects. Soybean plants were treated with two different sounds: chewing herbivore and forest ambient. The responses were markedly distinct, indicating that sound signals may also trigger specific cascades. Enzymes involved in oxidative metabolism were responsive to both sounds, while salicylic acid (SA) was responsive only to the chewing sound. In contrast, lipoxygenase (LOX) activity and jasmonic acid (JA) did not change. Soybean Kunitz trypsin inhibitor gene (SKTI) and Bowman-Birk (BBI) genes, encoding for protease inhibitors, were induced by chewing sound. Chewing sound-induced high expression of the pathogenesis-related protein (PR1) gene, confirming the activation of SA-dependent cascades. In contrast, the sound treatments promoted modifications in different branches of the phenylpropanoid pathway, highlighting a tendency for increased flavonols for plants under chewing sounds. Accordingly, chewing sounds induced pathogenesis-related protein (PR10/Bet v-1) and gmFLS1 flavonol synthase (FLS1) genes involved in flavonoid biosynthesis and flavonols. Finally, our results propose that plants may recognize herbivores by their chewing sound and that different ecological sounds can trigger distinct signaling cascades.},
DOI = {10.32604/phyton.2025.064068}
}