@Article{phyton.2025.068133,
AUTHOR = {Lina Song, Caijie Yi, Shiwei Zhao, Yuxin Peng, Zijing Li, Yuqiang Zhang , Hua Zhang, Helan Qin, Huali Zhang},
TITLE = {Comparative Transcriptomic Analysis of a Naturally Found Yellowish Leaf <i>Rehmannia chingii</i> H. L. Li Mutant and Wild Type},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {94},
YEAR = {2025},
NUMBER = {8},
PAGES = {2593--2613},
URL = {http://www.techscience.com/phyton/v94n8/63687},
ISSN = {1851-5657},
ABSTRACT = {Naturally occurring yellow leaf mutants are an important resource for studying pigment content and biosynthesis, as well as related gene expression. In our ongoing cultivation of <i>Rehmannia chingii</i> H. L. Li, we found an off-type yellow plant. The yellowing started with the new leaves and gradually spread downward until the entire plant exhibited a stable shade of yellow. We studied the differences in the chlorophyll and carotenoid content, carotenoid profile, and transcriptome of this yellow-leaf mutant (P2). Compared to the wild-type <i>R. chingii</i> plant (P1), P2 leaves had significantly lower chlorophyll and carotenoid content. LC-MS/MS analysis revealed that P2 had higher quantities of several metabolites in the carotenoid biosynthesis pathway. Transcriptome sequencing results showed that genes involved in porphyrin metabolism, carbon fixation, photosynthesis and antenna proteins, terpenoid backbone biosynthesis, and carotenoid biosynthesis were differentially expressed between P1 and P2. Large-scale expression differences were observed in the phytohormone and MAPK signaling pathways, as well as in 15 transcription factor families. We discuss possible mechanisms responsible for the yellow leaf color in P2. These preliminary data are valuable for further exploring the molecular mechanisms of leaf color formation and associated pathways.},
DOI = {10.32604/phyton.2025.068133}
}