@Article{phyton.2025.070017,
AUTHOR = {Jing Zhang, Huifen Qiao, Shenglong Wang, Jiawei Yuan, Qingsong Ba, Gensheng Zhang, Guiping Li},
TITLE = {Transcriptomics Provides New Insights into Resistance Mechanisms in Wheat Infected with <i>Puccinia striiformis</i> f. sp. <i>tritici</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {94},
YEAR = {2025},
NUMBER = {9},
PAGES = {2701--2718},
URL = {http://www.techscience.com/phyton/v94n9/63934},
ISSN = {1851-5657},
ABSTRACT = {Wheat stripe rust, a devastating disease caused by the fungal pathogen <i>Puccinia striiformis</i> f. sp. <i>tritici</i> (<i>Pst</i>), poses a significant threat to global wheat production. Growing resistant cultivars is a crucial strategy for wheat stripe rust management. However, the underlying molecular mechanisms of wheat resistance to <i>Pst</i> remain incompletely understood. To unravel these mechanisms, we employed high-throughput RNA sequencing (RNA-Seq) to analyze the transcriptome of the resistant wheat cultivar Mianmai 46 (MM46) at different time points (24, 48, and 96 h) post-inoculation with the <i>Pst</i> race CYR33. The analysis revealed that <i>Pst</i> infection significantly altered the expression of genes involved in photosynthesis and energy metabolism, suggesting a disruption of host cellular processes. Conversely, the expression of several resistance genes was upregulated, indicating activation of defense responses. Further analysis identified transcription factors (TFs), pathogen-related (PR) proteins, and chitinase-encoding genes as key players in wheat resistance to <i>Pst</i>. These genes likely contribute to the activation of defense pathways, such as the oxidative burst, which involves the production of reactive oxygen species (ROS). The activities of antioxidant enzymes, including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT), were also upregulated, suggesting a role in mitigating oxidative damage caused by ROS. Our findings provide valuable insights into the molecular mechanisms underlying wheat resistance to <i>Pst</i>. By identifying key genes and pathways involved in this complex interaction, we can develop more effective strategies for breeding resistant wheat cultivars and managing this destructive disease.},
DOI = {10.32604/phyton.2025.070017}
}