TY  - EJOU
AU  - Zhang, Jing 
AU  - Qiao, Huifen 
AU  - Wang, Shenglong 
AU  - Yuan, Jiawei 
AU  - Ba, Qingsong 
AU  - Zhang, Gensheng 
AU  - Li, Guiping 

TI  - Transcriptomics Provides New Insights into Resistance Mechanisms in Wheat Infected with <i>Puccinia striiformis</i> f. sp. <i>tritici</i>
T2  - Phyton-International Journal of Experimental Botany

PY  - 2025
VL  - 94
IS  - 9
SN  - 1851-5657

AB  - Wheat stripe rust, a devastating disease caused by the fungal pathogen <i>Puccinia striiformis</i> f. sp. <i>tritici</i> (<i>Pst</i>), poses a significant threat to global wheat production. Growing resistant cultivars is a crucial strategy for wheat stripe rust management. However, the underlying molecular mechanisms of wheat resistance to <i>Pst</i> remain incompletely understood. To unravel these mechanisms, we employed high-throughput RNA sequencing (RNA-Seq) to analyze the transcriptome of the resistant wheat cultivar Mianmai 46 (MM46) at different time points (24, 48, and 96 h) post-inoculation with the <i>Pst</i> race CYR33. The analysis revealed that <i>Pst</i> infection significantly altered the expression of genes involved in photosynthesis and energy metabolism, suggesting a disruption of host cellular processes. Conversely, the expression of several resistance genes was upregulated, indicating activation of defense responses. Further analysis identified transcription factors (TFs), pathogen-related (PR) proteins, and chitinase-encoding genes as key players in wheat resistance to <i>Pst</i>. These genes likely contribute to the activation of defense pathways, such as the oxidative burst, which involves the production of reactive oxygen species (ROS). The activities of antioxidant enzymes, including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT), were also upregulated, suggesting a role in mitigating oxidative damage caused by ROS. Our findings provide valuable insights into the molecular mechanisms underlying wheat resistance to <i>Pst</i>. By identifying key genes and pathways involved in this complex interaction, we can develop more effective strategies for breeding resistant wheat cultivars and managing this destructive disease.
KW  - Transcriptome analysis; wheat; yellow rust; antioxidant enzymes

DO  - 10.32604/phyton.2025.070017