@Article{phyton.2026.076015,
AUTHOR = {Haofan Lv, Qihang Zhang, Yufan He, Wuwei Xin, Wei Liu, Chunpeng Wan},
TITLE = {Investigation on Chemical Constituents from <i>Prunus cerasifera</i> Ehrh. Fruits and Evaluation of Their Anti-Inflammatory Activity},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {95},
YEAR = {2026},
NUMBER = {4},
PAGES = {--},
URL = {http://www.techscience.com/phyton/v95n4/67199},
ISSN = {1851-5657},
ABSTRACT = {The fruits of <i>Prunus cerasifera</i> Ehrh. have been traditionally utilized as both medicinal and edible resource, however, their specific phytochemical profile and anti-inflammatory mechanisms remain to be fully elucidated. This study aimed to isolate and identify the chemical constituents from the fruits and evaluate their anti-inflammatory activities. The separation was performed using a combination of chromatographic techniques. The structures of the obtained compounds were elucidated using a combination of <sup>1</sup>H and <sup>13</sup>C nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESI-MS). The anti-inflammatory activity of the compounds was initially investigated based on their capacity to inhibit nitric oxide (NO) release from lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and further validated in a zebrafish inflammatory model. The results indicated that eight compounds were successfully isolated from <i>Prunus cerasifera</i> Ehrh. fruits for the first time, including ursolic acid (<b>1</b>), corosolic acid (<b>2</b>), oleanolic acid (<b>3</b>), maslinic acid (<b>4</b>), 2-<i>O</i>-(3′,4′-dihydroxybenzoyl)-2,4,6-trihydroxyphenylacetic acid (<b>5</b>), neochlorogenic acid (<b>6</b>), chlorogenic acid (<b>7</b>), 3-<i>O</i>-<i>p</i>-coumaroylquinic acid (8). In the LPS-induced RAW264.7 cell model, compounds <b>6</b> and <b>7</b> exhibited stronger activity than the positive control dexamethasone (Dex, IC<sub>50</sub> = 11.13 μM ± 0.43 μM), with IC<sub>50</sub> values of 6.33 μM ± 0.18 μM and 8.06 μM ± 0.35 μM, respectively. In the LPS-induced zebrafish inflammation model, compounds <b>6</b> and <b>7</b> again demonstrated significant efficacy, exerting their anti-inflammatory effect primarily through the modulation of pro-inflammatory factors Interleukin-6 (IL-6) and Interleukin-1<i>β</i> (IL-1<i>β</i>).},
DOI = {10.32604/phyton.2026.076015}
}