TY  - EJOU
AU  - Al-Maaqar, Saleh 
AU  - Al-Johny, Bassam 
AU  - Al-Shaeri, Majed 
AU  - Al-Johny, Lara 
AU  - Qumusani, Adel 
AU  - Albalawy, Zakia 
AU  - Namla, Djadjiti 

TI  - Phytochemical Landscape of <i>Coleus forskohlii</i> and Its Role in Countering Staphylococcus Species
T2  - Phyton-International Journal of Experimental Botany

PY  - 2026
VL  - 95
IS  - 4
SN  - 1851-5657

AB  - Staphylococcal meningitis, a severe infection of the meninges, highlights the urgent need for new strategies to combat <i>Staphylococcus aureus</i> (<i>S. aureus</i>) infections. In this study, ethanolic leaf extracts of <i>Coleus forskohlii</i> were evaluated for their antibacterial potential against clinical <i>S. aureus</i> isolates associated with meningitis. Gas chromatography-mass spectrometry (GC-MS) analysis identified 15 phytochemical compounds, two of which—urs-12-en-28-ol (CID 22213452) and petroselaidic acid (CID 5282754) showed promising binding affinities (−7.5 and −5.9 kcal/mol, respectively) against <i>S. aureus</i> protein (30S ribosomal subunit) in molecular docking studies. <i>In vitro</i> assays confirmed the antibacterial activity of the crude extract, with a minimum inhibitory concentration (MIC) ranging from 62.5 to 125 μg/mL. Disc diffusion and time-kill kinetics further demonstrated concentration-dependent growth inhibition of <i>S. aureus</i> strains. These integrated findings suggest that <i>C. forskohlii</i>-derived compounds are potential antibacterial candidates worthy of further investigation. However, comprehensive <i>in vivo</i> studies are essential to evaluate their efficacy, safety, and therapeutic potential specifically against <i>S. aureus</i>-induced meningitis.
KW  - <i>Coleus forskohlii</i>; GC-MS; meningitis; antibacterial; bacteria; virtual screening; ADMET

DO  - 10.32604/phyton.2026.077998