@Article{phyton.2026.078590,
AUTHOR = {Shengqun Chen, Lianwen Shen, Shuang Qu, Xia Jiang, Gang Wang},
TITLE = {Integrated Transcriptome and Lipidome to Analyze the Characteristics of Oil Accumulation in Seeds of <i>Acer truncatum</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {95},
YEAR = {2026},
NUMBER = {4},
PAGES = {--},
URL = {http://www.techscience.com/phyton/v95n4/67208},
ISSN = {1851-5657},
ABSTRACT = {<i>Acer truncatum</i> is a significant woody oil-bearing tree species known for its ability to synthesize various unsaturated fatty acids. This study systematically analyzes the lipid metabolic pathways and the associated transcript abundance changes involved in the biosynthesis and accumulation of seed oil in <i>A. truncatum</i>. By integrating lipidomics and transcriptomics analyses across different developmental stages of <i>A. truncatum</i> seeds, we thoroughly investigate the dynamic characteristics of oil metabolism. The results show that triacylglycerols (TAGs) become the dominating lipid class throughout seed development and that their amount increases greatly as the seeds mature, whereas diacylglycerols (DAGs) show a significantly decreased relative abundance. Numerous differentially expressed genes (DEGs) linked to lipid biosynthesis were identified by transcriptome analysis, including <i>AcACCase</i>, <i>AcKAS</i>, <i>AcKCS</i>, <i>AcGPAT</i>, <i>AcDGAT</i>, and <i>AcOLE</i>. The expression patterns of DGAT- and KCS-encoding genes differ noticeably between immature and mature seeds. Integrated lipidomics and transcriptomics analyses suggest stage-associated lipid metabolic changes during seed development of <i>A. truncatum</i>. This study also highlights candidate genes and metabolic pathways with TAG accumulation. These findings improve our understanding of seed oil metabolism in <i>A. truncatum</i> by linking <i>Ac</i>DGAT-related TAG assembly and <i>Ac</i>KCS-driven very-long-chain fatty acid elongation to the developmental regulation of the Kennedy pathway (TAG biosynthesis) and fatty acid elongation (VLCFA/NA-related), providing essential molecular insights for the genetic improvement of oil yield and fatty acid quality in this species.},
DOI = {10.32604/phyton.2026.078590}
}