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  • Open Access

    ARTICLE

    Taxus globosa S. cell lines: Initiation, selection and characterization in terms of growth, and of baccatin III and paclitaxel production

    DULCE MA. BARRADAS-DERMITZ1,*, PATRICIA M. HAYWARD-JONES2, MARTÍN MATA-ROSAS3, BEATRIZ PALMEROSSÁNCHEZ2, OSCAR B. J. PLATAS-BARRADAS4, RODOLFO F. VELÁSQUEZ-TOLEDO2

    BIOCELL, Vol.34, No.1, pp. 1-6, 2010, DOI:10.32604/biocell.2010.34.001

    Abstract Of the initial six cell lines originating from explants of Taxus globosa, or Mexican yew (stem internode, leaves and meristematic tissue), three were selected for their microbial and oxidation resistance, two from leaves and the other from stem internode. A study of their behavior, both in terms of cell growth, and of baccatin III and paclitaxel production, was developed in suspension cultures with an initially standardized biomass (fresh weight 0.23 g/L) using modified Gamborg’s B5 medium, and an elicitor (methyl jasmonate), on either the first or seventh day of culture, at several levels (0, 0.1, 1, More >

  • Open Access

    ARTICLE

    Plant regeneration of Mullein Nightshade (Solanum donianum Walp.) from leaf explants

    O’Connor-Sánchez1 A, AV Domínguez-May1, MA Keb-Llanes1, YJ Peña-Ramírez2, VA Herrera-Valencia1

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 25-29, 2010, DOI:10.32604/phyton.2010.79.025

    Abstract A reliable protocol for plant regeneration of Mullein Nightshade (Solanum donianum Walp.) was developed from in vitro leaf explants. They were cultured on Murashige and Skoog semisolid medium, supplemented with several combinations of zeatin riboside and 1-naphthaleneacetic acid for shoot regeneration. The formulation that originated most shoots per explant was 8.5 μM zeatin riboside in absence of 1-naphthalene acetic acid. Explants with shoots were transferred to Murashige and Skoog medium, with half the normal salt concentration and without plant growth regulators for elongation. Elongated shoots were individualized, and they rooted readily in half-strength Murashige and Skoog More >

  • Open Access

    ARTICLE

    West Indian avocado: where did it originate?

    Galindo-Tovar1 ME, AM Arzate-Fernández2

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 203-207, 2010, DOI:10.32604/phyton.2010.79.203

    Abstract Origin of West Indian avocado has been explained by current-day factors, such as abundance patterns; however, it is still unclear where it originated. Anyhow, there is a history behind the species that reflects, in one way or another, the present geographic distribution and species characteristics. We examined paleohistorical and paleoecological data to explore the West Indian avocado origin. Our results suggest that West Indian avocado originated by domestication and ecological adaptation in the Maya Lowland area; this is where this botanical variety of avocado has an ancient history. More >

  • Open Access

    ARTICLE

    Records of Sclerotinia minor infecting cultivated and invasive plants in the southern Pampas and northern Patagonia, Argentina, including comparative studies with Sclerotinia sclerotiorum

    Kiehr M1, R Delhey1, J Lusto2, PJ Paoloni3

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 199-202, 2010, DOI:10.32604/phyton.2010.79.199

    Abstract A report is given on host plants, distribution, damage and importance of Sclerotinia minor in the southern Pampas and northern Patagonia regions (Argentina). Spontaneous infections were recorded on seven plant species. Sonchus oleraceus and Eruca vesicaria are new hosts for S. minor; Cichorium intybus and Zinnia elegans are new hosts for Argentina. This fungus causes important damage on Helianthus annuus and Lactuca sativa, and it occasionally affects C. intybus and Solanum lycopersicum. Comparative in vitro and in planta studies were carried out with S. minor and S. sclerotiorum isolates obtained from sunflower. More >

  • Open Access

    ARTICLE

    Osmotic adjustment in Leymus cinereus cv. “Trailhead” under field conditions

    Torres1 YA, CA Busso1, OA Montenegro2, HD Giorgetti2, HD Rodríguez2, D Bentivegna3

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 195-198, 2010, DOI:10.32604/phyton.2010.79.195

    Abstract Osmotic adjustment has been recognized to be an important adaptive response to water stress in higher plants. Although Leymus cinereus cv. “Trailhead” is reputedly a very drought-tolerant rangeland plant, its ability to adjust osmotically in the field has not previously been reported. This study examined the occurrence or not of osmotic adjustment in field-grown plants of Leymus cinereus cv. “Trailhead” during late spring in an arid zone of Patagonia. Plants of this species had been established in the field three years before. With this purpose, leaf laminae of this species were collected and pressure-volume curves made. Results More >

  • Open Access

    ARTICLE

    Wild vegetable use by Vhavenda in the Venda region of Limpopo Province, South Africa

    Maanda MQ, RB Bhat

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 189-194, 2010, DOI:10.32604/phyton.2010.79.189

    Abstract We studied the marginal utilization of 40 wild vegetable species in the Venda area of Limpopo Province (South Africa). Use of these vegetables with various purposes by humans is indicated. More >

  • Open Access

    ARTICLE

    Relationship between leaf greenness index and leaf water status in wheat under different tillage systems

    Mirassón1 HR, ML Faraldo1, MN Fioretti2, M Miravalles2, RE Brevedan2

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 183-187, 2010, DOI:10.32604/phyton.2010.79.183

    Abstract Tillage system is an important management decision that has to be taken in the central, semiarid, temperate region of Argentina for dryland wheat production. A 2-year study was conducted on an Entic Haplustoll soil with a sandy loam texture to determine the effects of three tillage systems (conventional, vertical and notillage) on leaf water status and chlorophyll content. Measurements were made at the Z65 growth stage with a SPAD-502; leaf water potential measurements were simultaneously taken. There was a positive relationship between leaf water potential and leaf greenness in both years. Tillage systems were significantly More >

  • Open Access

    ARTICLE

    An efficient protocol for culturing meristems of sorghum hybrids

    Sadia1,3 B, PC Josekutty2, SD Potlakayala2, P Patel2, S Goldman3, SV Rudrabhatla2

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 177-181, 2010, DOI:10.32604/phyton.2010.79.177

    Abstract A robust protocol for culturing meristems of Sorghum is required to assist with rapid genetic improvement of the genus. Through meristem culture, an efficient method for rapid micropropagation was developed for Sorghum bicolor (L.) Moench. hybrids, namely NC+262, NC+6C21 and NC+6B50. Complete plants were regenerated directly from shoot meristems without an intervening callus phase. Regeneration frequencies varied between the studied genotypes and according to the growth regulator combinations present in the medium. The combination of BAP and TDZ showed a synergistic effect on shoot multiplication. The highest number of shoots per meristem (68 ± 2) was recorded More >

  • Open Access

    ARTICLE

    Vegetation response to a controlled fire in the Phytogeographical Province of the Monte, Argentina

    Peláez1 DV, HD Giorgetti2, OA Montenegro2, OR Elia3, GD Rodríguez2, RM Bóo1,*, MD Mayor4,*,*, CA Busso3

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 169-176, 2010, DOI:10.32604/phyton.2010.79.169

    Abstract Fire is a major ecological process within most terrestrial ecosystems. Therefore, understanding the vegetation response to fire is essential to its management. Our objective was to study the effect of a controlled fire, conducted at the end of summer season, on cover and density of the most common woody and herbaceous species in Phytogeographical Province of the Monte, Argentina. In general, after the control fire, the woody species mean percentage cover was significantly lower (p<0.05) in the fire treatment than in the control (no controlled fire). This effect was persistent during the study period. No… More >

  • Open Access

    ARTICLE

    Cloning and expression analysis of rubredoxin from cold-treated banana leaves

    Feng1 RJ, LF Lu2, KH Yuan3, P Cheng3, LL Zhang3, JF Qi3, Y Ren4, XL Xu5, XB Zhang3, LY Zhou3, YD Zhang3

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 163-168, 2010, DOI:10.32604/phyton.2010.79.163

    Abstract A banana (Musa AAA, Cavendish subgroup cv. Brazil) cDNA encoding a putative rubredoxin-like protein (MaRd1) was obtained from total RNA isolated from cold-treated banana leaves using rapid amplification of cDNA ends (RACE) technique. MaRd1 cDNA contained 597 nucleotides encoding 198 amino acids in the open reading frame. MaRd1 protein showed 56% amino acid identity with that of Pyrococcus furiosus rubredoxin (P24297). A chloroplast transit peptide and a transmembrane region were detected at the N-terminus and the C-terminus, respectively, of the deduced amino acid sequence of MaRd1 gene. Southern blotting revealed the occurrence of at least two copies of MaRd1 More >

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