Home / Advanced Search

  • Title/Keywords

  • Author/Affliations

  • Journal

  • Article Type

  • Start Year

  • End Year

Update SearchingClear
  • Articles
  • Online
Search Results (5)
  • Open Access


    Doubled Haploid Production Using an Improved Anther Culture Protocol for Sorghum [Sorghum bicolor (L.) Moench]

    Paul Chege1,2, Erzsébet Kiss2, Csaba Lantos1, Andrea Palágyi1, János Pauk1,2,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 475-487, 2021, DOI:10.32604/phyton.2021.013557

    Abstract Sorghum [Sorghum bicolor (L.) Moench] can benefit from accelerated breeding and release of improved varieties through doubled haploid technology. The technology has been used in speeding up the breeding of other major cereals such as wheat, maize and rice, for which generally widely applied optimised protocols exist. A reproducible protocol for the crop, that can overcome genotype dependency and other species-specific challenges such as phenolic exudation is however lacking. This study aimed at sorghum doubled haploids production thereby contributing to the development of an improved protocol. From the 28 hybrid genotypes, both F1 registered- and experimental hybrids involved, this study… More >

  • Open Access


    Improved Culture Media for Embryogenic Callus Generation in Sorghum [Sorghum bicolor (L.) Moench]

    Paul Chege1,2, Andrea Palágyi2, Csaba Lantos2, Erzsébet Kiss1, János Pauk2,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 111-119, 2020, DOI:10.32604/phyton.2020.07554

    Abstract Many attempts on optimization of sorghum [Sorghum bicolor (L.) Moench] tissue culture induction media have been made, but the culture system remains with some bottlenecks compared to that of other crops. This study aimed at assessing the suitability of various induction media to produce embryogenic callus (yellow and friable) with high induction rates and reduced phenolic exudation. The six culture medium modifications: 3 based on Murashige and Skoog (MS) medium and one each based on Chu N6, Gamborg B5 and 190-2 media respectively were applied in the culture of mature embryos from 10 sorghum genotypes. Although there was a genotype… More >

  • Open Access


    Biomass production and grain yield of three sorghum lines differing in drought resistance

    Castro-Nava S1, J Ortiz-Cereceres2,†, M del C Mendoza-Castillo2, AJ Huerta3

    Phyton-International Journal of Experimental Botany, Vol.81, pp. 149-156, 2012, DOI:10.32604/phyton.2012.81.149

    Abstract The aim of this study was to determine the efficiency of drought classification criteria that we previously reported for our grain sorghum genotypes. Two tolerant genotypes of grain sorghum [Sorghum bicolor (L.) Moench] (UAT-124 and UAT-152) and one susceptible (UAT-30) were subjected to drought under greenhouse conditions at either panicle initiation, flag leaf, or flowering. Results showed that the effects of drought depended on when drought stress occurred during development. Biomass of the three sorghum genotypes was significantly reduced at the flag leaf stage (48%) by drought stress, but the greatest reduction occurred in the susceptible genotype UAT-30 (71%). Results… More >

  • Open Access


    An efficient protocol for culturing meristems of sorghum hybrids

    Sadia1,3 B, PC Josekutty2, SD Potlakayala2, P Patel2, S Goldman3, SV Rudrabhatla2

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 177-181, 2010, DOI:10.32604/phyton.2010.79.177

    Abstract A robust protocol for culturing meristems of Sorghum is required to assist with rapid genetic improvement of the genus. Through meristem culture, an efficient method for rapid micropropagation was developed for Sorghum bicolor (L.) Moench. hybrids, namely NC+262, NC+6C21 and NC+6B50. Complete plants were regenerated directly from shoot meristems without an intervening callus phase. Regeneration frequencies varied between the studied genotypes and according to the growth regulator combinations present in the medium. The combination of BAP and TDZ showed a synergistic effect on shoot multiplication. The highest number of shoots per meristem (68 ± 2) was recorded for the genotype… More >

  • Open Access


    Somatic embryogenesis induction from immature embryos of Sorghum bicolor L. (Moench)

    Espinoza-Sánchez EA1, YA Sánchez-Peña2, JA Torres-Castillo3, EA García-Zambrano2, JE Treviño Ramírez2, F Zavala-García2, SR Sinagawa-García2

    Phyton-International Journal of Experimental Botany, Vol.87, pp. 105-112, 2018, DOI:10.32604/phyton.2018.87.105

    Abstract In this study, we established an in vitro regeneration system from immature embryos of four varieties of Sweet Sorghum with biotechnological potential. A total of 17 treatments were assesses in the induction of somatic embryogenic calli. We analyzed the effect of Murashige y Skoog media supplemented with different concentrations of plant growth regulators such as auxins and cytokinins. The callus induction response was evaluated using three immature embryos development stages and based on the number of embryos with callus induction. Different responses were observed in the embryos depending of the embryo age, culture condition, media composition and variety. The results… More >

Displaying 1-10 on page 1 of 5. Per Page  

Share Link