M.T. MARTIN*, H.E. PEDRANZANI**, R. SIERRA DE GRADO
BIOCELL, Vol.31, No.1, pp. 41-49, 2007, DOI:10.32604/biocell.2007.31.041
Abstract An in vitro collection has been established with selected European aspen from Palencia province (Spain). Currently, this collection includes 32 high quality clones, selected for their good bearing and
healthy state. Most of them belong to different discrete local populations.
Populus tremula L. was propagated in proliferation Aspen Culture Medium; they required subculture every
3 months. The purpose of this study was, therefore, to select a medium which allows the maintenance of 32
clones for a period longer than 3 months without subculture and to observe the behavior of those clones in 15
different culture medium compositions. Seven… More >