Zhiguo Wang^*†1, Zehui Fang^‡1, Runzhang Lu^†, Hongli Zhao^‡, Tiejun Gong^†, Dong Liu^†, Luojia Hong^‡, Jun Ma^†, Mei Zhang^*

Oncology Research, Vol.27, No.9, pp. 1035-1042, 2019, DOI:10.3727/096504019X15528367532612

Abstract Although arsenic trioxide (ATO) is a well-known antileukemic drug used for acute promyelocytic leukemia treatment, the development of ATO resistance is still a big challenge. We previously reported that microRNA- 204 (miR-204) was involved in the regulation of acute myeloid leukemia (AML) cell apoptosis, but its role in chemoresistance is poorly understood. In the present study, we showed that miR-204 was significantly increased in AML cells after ATO treatment. Interestingly, the increased miR-204 level that was negatively correlated with ATO induced the decrease in cell viability and baculoviral inhibition of apoptosis protein repeatcontaining 6 (BIRC6)… More >

Juanjuan Yao^*, Liang Zhong^†, Pengqiang Zhong^*, Dongdong Liu^†, Zhen Yuan^†, Junmei Liu^*, Shifei Yao^*, Yi Zhao^*, Min Chen^*, Lianwen Li^*, Lu Liu^†, Beizhong Liu^*†

Oncology Research, Vol.27, No.7, pp. 809-818, 2019, DOI:10.3727/096504018X15451301487729

Abstract RAS-responsive element-binding protein 1 (RREB1) is a transcription factor that is implicated in RAS signaling and multiple tumors. However, the role of RREB1 in acute myeloid leukemia has not been studied. We found that RREB1 is overexpressed in AML patients and myeloid leukemia cell lines (NB4 and HL-60), and RREB1 expression was significantly decreased during granulocytic differentiation of myeloid leukemia cells induced by all-trans retinoic acid (ATRA). Then we performed a RREB1 knockdown assay in NB4 and HL-60 cells; the results showed that knockdown of RREB1 upregulated expression of CD11b, CEBP , and microRNA-145 (miR-145), More >

Hong Li^*1, Yaning Tian^*1, Xiang Li^*, Bin Wang^†, Dongzhi Zhai^*, Yingying Bai^*, Changhu Dong^*, Xu Chao^*‡

Oncology Research, Vol.27, No.6, pp. 673-680, 2019, DOI:10.3727/096504018X15426261956343

Abstract IARS2 encodes mitochondrial isoleucine-tRNA synthetase, which mutation may cause multiple diseases. However, the biological function of IARS2 on acute myeloid leukemia (AML) has not yet been identified. In the present study, qRT-PCR was used to determine the expression of IARS2 in K562, THP1, and HL-60 leukemia cells. Additionally the mRNA levels of IARS2 in CD34 cells and AML cells obtained from patients were detected by qRT-PCR. IARS2-shRNA lentiviral vector was established and used to infect acute myeloid leukemia HL-60 cells. qRT-PCR and Western blot analysis were employed to assess the knockdown effect of IARS2. The… More >

Angran Li¹, Xiaoqi Chai², Ge Yang^2,3, Yongjie Jessica Zhang^1,2,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 66-66, 2019, DOI:10.32604/mcb.2019.07633

Abstract Neurons exhibit remarkably complex geometry in their neurite networks. So far, how materials are transported in the complex geometry for survival and function of neurons remains an unanswered question. Answering this question is fundamental to understanding the physiology and disease of neurons. Here, we have developed an isogeometric analysis (IGA) based platform for material transport simulation in neurite networks. We modeled the transport process by reaction-diffusion-transport equations and represented geometry of the networks using truncated hierarchical tricubic B-splines (THB-spline3D). We solved the Navier-Stokes equations to obtain the velocity field of material transport in the networks. More >

Angran Li¹, Xiaoqi Chai², Ge Yang^2,3, Yongjie Jessica Zhang^1,2,*

Molecular & Cellular Biomechanics, Vol.16, No.2, pp. 123-140, 2019, DOI:10.32604/mcb.2019.06479

Abstract Neurons exhibit remarkably complex geometry in their neurite networks. So far, how materials are transported in the complex geometry for survival and function of neurons remains an unanswered question. Answering this question is fundamental to understanding the physiology and disease of neurons. Here, we have developed an isogeometric analysis (IGA) based platform for material transport simulation in neurite networks. We modeled the transport process by reaction-diffusion-transport equations and represented geometry of the networks using truncated hierarchical tricubic B-splines (THB-spline3D). We solved the Navier-Stokes equations to obtain the velocity field of material transport in the networks. More >

Ericka Sohlberg, Andrew Sun, Rustin Massoudi, Kris Prado, Eila Skinner

Canadian Journal of Urology, Vol.25, No.6, pp. 9614-9616, 2018

Abstract While renal angiomyolipomas (AMLs) generally remain small and asymptomatic, larger AMLs are more common in tuberous sclerosis patients. Giant AMLs over 20 cm are a rare entity and little is known about their management. We present a unique case of a 48-year-old woman with tuberous sclerosis and a 39 cm AML arising from a solitary kidney, after undergoing nephrectomy for a prior AML. Giant renal AMLs can occur in patients with tuberous sclerosis and resection should be considered even for large tumors. Renal sparing is often difficult and patients should be counseled about potential need More >

Yueli Gu^*, Jinchun Si^†, Xichun Xiao^*, Ying Tian^*, Shuo Yang^*

Oncology Research, Vol.25, No.7, pp. 1069-1079, 2017, DOI:10.3727/096504016X14829256525028

Abstract Aberrant expression of microRNA-92a (miR-92a) has been investigated in various cancers. However, the function and mechanism of miR-92a in acute myeloid leukemia (AML) remain to be elucidated. Our data showed that miR-92a was evidently downregulated and methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) was remarkably upregulated in AML cell lines HL-60 and THP-1. Dual luciferase reporter assay revealed that MTHFD2 was a direct target of miR-92a. Gain- and loss-of-function analysis demonstrated that MTHFD2 knockdown or miR-92a overexpression notably inhibited proliferation and promoted apoptosis of AML cell lines. Restoration of MTHFD2 expression reversed proliferation inhibition and apoptosis induction of More >

Hoi Lam She^*, Arno A.W. Roest^†, Emmeline E. Calkoen^†, Pieter J. van den Boogaard^‡, Rob J. van der Geest^‡, Mark G. Hazekamp^§, Albert de Roos^‡, Jos J.M. Westenberg^‡

Congenital Heart Disease, Vol.12, No.1, pp. 40-48, 2017

Abstract Purpose. To evaluate the inflow pattern and flow quantification in patients with functional univentricular heart after Fontan’s operation using 4D flow magnetic resonance imaging (MRI) with streamline visualization when compared with the conventional 2D flow approach.
Method. Seven patients with functional univentricular heart after Fontan’s operation and twenty-three healthy controls underwent 4D flow MRI. In two orthogonal two-chamber planes, streamline visualization was applied, and inflow angles with peak inflow velocity (PIV) were measured. Transatrioventricular flow quantification was assessed using conventional 2D multiplanar reformation (MPR) and 4D MPR tracking the annulus and perpendicular to the streamline inflow at… More >

Ping Chen^*, Qing Jin^*, Qiang Fu^*, Peidong You^*, Xi Jiang^*, Qin Yuan^*, Huifang Huang^†

Oncology Research, Vol.24, No.4, pp. 215-223, 2016, DOI:10.3727/096504016X14634208143021

Abstract This study aimed to investigate the role of the PI3K/Akt signaling pathway in multidrug resistance of acute myeloid leukemia (AML) cells induced by cocultured stromal cells. Human AML cell lines HL-60 and U937 were adhesion cocultured with human bone marrow stromal cell line HS-5 cells. Such coculturing induced HL-60 and U937 cells resistant to chemotherapeutic drugs including daunorubicin (DNR), homoharringtonine (HHT), and cytosine arabinoside (Ara-C). The coculturing-induced resistance of AML cells to DNR, HHT, and Ara-C can be partially reversed by inhibition of the PI3K/Akt signaling pathway. Clinically, AML patients with a low level of More >

Gangcan Li, Yanping Song, Yunjie Zhang, Hao Wang, Jia Xie

Oncology Research, Vol.24, No.2, pp. 109-116, 2016, DOI:10.3727/096504016X14611963142254

Abstract Acute myeloid leukemia (AML) is the most lethal hematological malignancy, and the occurrence of chemoresistance prevents the achievement of complete remission following the standard therapy. MicroRNAs have been extensively investigated as critical regulators of hematopoiesis and leukemogenesis, and they represent a promising strategy for AML therapy. In this study, we identified miR-34b as a novel regulator in myeloid proliferation and apoptosis of leukemic cells. We found that miR-34b was developmentally upregulated in plasma and myeloid cells of healthy subjects, while it was significantly reduced in blood samples of patients with AML and AML cell lines.… More >