Cohen¹ G, L Mascarini¹, CC Xifreda²

Phyton-International Journal of Experimental Botany, Vol.81, pp. 199-204, 2012, DOI:10.32604/phyton.2012.81.199

Abstract Micromorphological and anatomical differences in leaf epidermis and stem xylem of Rosa hybrida ‘Lovely Red ‘ (LR) and ‘Rouge Baiser’ (RB), commercially grown in Buenos Aires, Argentina, were examined in the lower half of the terminal leaflet of 5-foliate leaves and in the basal part of stem, respectively. Digital images obtained from SEM and a phase contrast microscope were used with this purpose. Epicuticular wax, which was present on both sides of the leaves, was more abundant in LR than in RB. Stomata were only present on the abaxial side; they were 111 and 129 per… More >

Valdés Oyervides FJ¹, C Rivas Morales², A Benavides Mendoza¹, MA Núñez González², J Verde Star², A Oranday Cárdenas², V Robledo Torres²

Phyton-International Journal of Experimental Botany, Vol.81, pp. 191-198, 2012, DOI:10.32604/phyton.2012.81.191

Abstract Four dynamic conditions to evaluate the effects of stress on production of biomass, essential oils, Thymol, carvacrol and proline accumulation in Lippia graveolens H.B.K. were investigated under greenhouse conditions. A bifactorial design assessed four environmental conditions (factor B): commercial nutritive solution (B0); Water (B1); moderate stress (B2), and intermediate (B3) stress nested under (Factor A) salinity (NaCl) and ion concentration (Cu²⁺) and (Fe²⁺) stresses. Average production of fresh weight and number of leaves were reduced under NaCl and both ion concentration conditions. However, there were no significant differences on average for the four environments. The amounts of… More >

De la Rosa M¹, L Arce¹, JA Villarreal¹, L Ibarra², J Lozano³

Phyton-International Journal of Experimental Botany, Vol.81, pp. 165-168, 2012, DOI:10.32604/phyton.2012.81.165

Abstract Simojovel pepper seeds were first treated with 0.5 M NaCl, and then put to germinate in solutions of gibberellic acid at different concentrations. Initially, seeds were either preconditioned or not with NaCl combined with 0, 100, 200, 300 or 400 mg/L gibberellic acid. In a second phase of the study, seeds preconditioned with NaCl were exposed to 0, 350, 400, 450 or 500 mg/L gibberellic acid. Experiments were arranged in a completely randomized design with a factorial arrangement of 2 x 5 with four replications. The greatest (p≤0.01) germination percentage (91.75%) was obtained on seeds More >

Kiehr M¹, R Delhey¹, A Azpilicueta²

Phyton-International Journal of Experimental Botany, Vol.81, pp. 161-164, 2012, DOI:10.32604/phyton.2012.81.161

Abstract While studying the etiology of onion diseases in southern Argentina, a coelomycetous fungus was found associated with diverse disease expressions. They included the typical smudge symptoms in white onion bulbs in local markets as well as field infections on yellow- and red-coloured cultivars, with severe infections on transplants in the seed-bed. The fungus was isolated and identified as Colletotrichum circinans on the basis of its morphological, morphometric, biological and pathological characteristics. Two isolates, obtained from white bulbs and from transplants, were inoculated on bulbs of white and yellow-coloured cultivars and produced the typical smudge symptoms on More >

Dhyani A¹, BP Nautiyal², MC Nautiyal¹, MC Rivera³, D Prasad⁴, KP Singh⁴

Phyton-International Journal of Experimental Botany, Vol.81, pp. 157-159, 2012, DOI:10.32604/phyton.2012.81.157

Abstract Lilium polyphyllum is a critically endangered herb in the wilds of Uttarakhand, India. Gray mold appeared as a new disease on this species from July to August (2008-2009) causing death of inflorescences and shoots. Botrytis cinerea was consistently isolated on PDA, from infected inflorescences. Pathogenicity was established by inoculation of potted plants of L. polyphyllum in a glasshouse. Botrytis cinerea was recorded as a gray mold pathogen on L. polyphyllum for the first time in India, causing flower and shoot blight. This disease may seriously affect reproductive stages of this endangered host. To our knowledge, B. cinerea had not been More >

Castro-Nava S¹, J Ortiz-Cereceres^2,†, M del C Mendoza-Castillo², AJ Huerta³

Phyton-International Journal of Experimental Botany, Vol.81, pp. 149-156, 2012, DOI:10.32604/phyton.2012.81.149

Abstract The aim of this study was to determine the efficiency of drought classification criteria that we previously reported for our grain sorghum genotypes. Two tolerant genotypes of grain sorghum [Sorghum bicolor (L.) Moench] (UAT-124 and UAT-152) and one susceptible (UAT-30) were subjected to drought under greenhouse conditions at either panicle initiation, flag leaf, or flowering. Results showed that the effects of drought depended on when drought stress occurred during development. Biomass of the three sorghum genotypes was significantly reduced at the flag leaf stage (48%) by drought stress, but the greatest reduction occurred in the susceptible… More >

Escalante-González JG¹, EA García-Zambrano², A Gutiérrez-Diez², RE Vázquez-Alvarado², JA Torres-Castillo², SR Sinagawa-García², F Zavala-García², J Martínez-de la Cerda², C Gallegos-Vázquez³, JI García-Zambrano⁴

Phyton-International Journal of Experimental Botany, Vol.81, pp. 143-148, 2012, DOI:10.32604/phyton.2012.81.143

Abstract The objective of this research was to increase the genetic diversity of the Nopal Germplasm Bank of the FAUANL using RAPD molecular markers to estimate the genetic diversity of 15 accessions of cactus pear from molecular markers recorded in the Bank. DNA extraction was performed by the method of cellular ruptor using FastDNA^® Kit. Performance measurement of DNA was carried out through Picoflour. It was observed that the DNA concentrations ranged from 74.91 ng/µL to 12.32 ng/uL. Twelve primers were used to generate from 1 to 5 bands per primer with a molecular weight ranging from More >

Hernández-León R¹, M Martínez-Trujillo², E Valencia-Cantero¹, G Santoyo¹

Phyton-International Journal of Experimental Botany, Vol.81, pp. 133-137, 2012, DOI:10.32604/phyton.2012.81.133

Abstract Rhizospheric soil of wheat plants contains a high diversity of microorganisms, and therefore, comprises a large reservoir for discovering genes with diverse agro-biotechnological applications. In this work, we constructed an E. coli metagenomic library based on bacterial artificial chromosome (BAC) clones with large genomic inserts from metagenomic DNA from the rhizosphere of wheat plants. The average of the DNA cloned segments varies from 5 to 80 kb, with an average size of 38 kb. Random clones were end-sequenced and homology results showed that the clonation of metagenomic DNA codes mainly for metabolic and catalytic functions (40%), More >

Borghi ML¹, MA Ibañez¹, NC Bonamico¹, MV Kandus², D Almorza Gomar³, EA Guillin², JC Salerno², MA Di Renzo¹

Phyton-International Journal of Experimental Botany, Vol.81, pp. 123-131, 2012, DOI:10.32604/phyton.2012.81.123

Abstract Mal de Río Cuarto (MRC) disease is a significant threat to corn production in Argentina. Sixty six hybrid combinations derived from the cross of twelve flint maize (Zea mays L) inbred lines were evaluated using a completely randomized block design with two replications. MRC disease severity grade (SG) and grain yield (GY) were the analyzed traits. Our objectives were to estimate the general (GCA) and specific (SCA) combining abilities for these traits among twelve corn inbred lines, and to identify potential heterotic relationships among them. Diallel crosses were performed and these results were compared using Griffing’s… More >

Flores-Naveda A, CGS Valdés-Lozano, WL Rooney, E Olivares-Sáenz, F Zavala-García, A Gutiérrez-Díez, ME Vázquez-Badillo

Phyton-International Journal of Experimental Botany, Vol.81, pp. 113-122, 2012, DOI:10.32604/phyton.2012.81.113

Abstract The objective of this work was to evaluate, under irrigation and limited irrigation, the grain yield of 40 F7 sorghum lines (Sorghum bicolor L. Moench) selected by tolerance to drought in the Facultad de Agronomía at the Universidad Autónoma de Nuevo León (FAUANL) in Marín, Nuevo León, Mexico. Studies were conducted in College Station, Texas in the cycle Autumn-Winter 2011, and in Lubbock, Texas in the growing cycle Spring-Summer 2011. The 40 sorghum lines were divided into two groups of 20 genotypes each to integrate two experiments, and five more experimental lines and a commercial More >