Home / Advanced Search

  • Title/Keywords

  • Author/Affliations

  • Journal

  • Article Type

  • Start Year

  • End Year

Update SearchingClear
  • Articles
  • Online
Search Results (10,856)
  • Open Access

    ARTICLE

    Ultrastructural effects of two phthalocyanines in CHO-K1 and HeLa cells after laser irradiation

    Marcelo de Castro Pazos*, Cristina Pacheco-Soares*, Newton Soares da Silva*, R.A. DaMatta**, M.T.T. Pacheco*

    BIOCELL, Vol.27, No.3, pp. 301-309, 2003, DOI:10.32604/biocell.2003.27.301

    Abstract The effects of Photodynamic Therapy using 2nd generation photosensitizers have been widely investigated aiming clinical application treatment of solid neoplasms. In this work, ultrastructure changes caused by the action of two 2nd generation photosensitizers and laser irradiation on CHO-K1 and HeLa (neoplastic) cells were analyzed by transmission electron microscopy. Aluminum phthalocyanine chloride, aluminum phthalocyanine tetrasulfonate chloride and radiation from a semiconductor laser at a fluency of 0.5 J/ cm2 (Power=26mW; λ=670nm) were used. The results showed induction of apoptosis. Such alterations where observed in HeLa but not in CHO-K1 cells after Aluminum phthalocyanine tetrasulfonate chloride (AlPcS4) photodynamic More >

  • Open Access

    ARTICLE

    Apoptogenic effect of the lipophilic o-naphthoquinone CG 10-248 on rat hepatocytes: light and electron microscopy studies

    Lidia M. Lopez*, Marta Dubin**, Patricia H. Carrizo**, Mario H. Burgos***, Amanda Pellegrino De Iraldi*, Andrés O. M. Stoppani**

    BIOCELL, Vol.27, No.2, pp. 213-224, 2003, DOI:10.32604/biocell.2003.27.213

    Abstract CG 10-248 (3,4-dihydro-2,2-dimethyl-9-chloro-2H-naphtho[1,2b]pyran- 5,6-dione; CG-NQ), a β-lapachone analogue, modified the ultrastructure of rat hepatocytes, as demonstrated by light and electron microscopy. After 4 h incubation with 100 µM CG-NQ, the following effects were observed: (a) nuclear chromatin condensation; (b) chromatin fragmentation; (c) displacement of mitochondria, concentrated around the nucleus; (d) disruption or expansion of mitochondrial outer or inner membranes, respectively; (e) displacement and alteration of endoplasmic reticulum (rough and smooth); (f) decrease of microvilli; (g) blebbing of plasma membrane and production of apoptotic bodies formed by folding of plasma membrane fragments around mitochondria or peroxysomes; More >

  • Open Access

    ARTICLE

    Fine structural study of the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta)

    José M. Estevez*, Eduardo J. Cáceres**

    BIOCELL, Vol.27, No.2, pp. 181-187, 2003, DOI:10.32604/biocell.2003.27.181

    Abstract The present study analyzed several characters of the red seaweed Gymnogongrus torulosus, such as cellular structure of the thallus, cuticle, pit plug and cell wall ultrastructure, and morphology of some organelles like plastids, Golgi bodies and mitochondria. Also, anomalous chloroplasts with thylakoid disorganization were found in medullary cells. The significance of this thylakoid disposition is still unclear. This is one of the first studies focused on the fine structure of a red alga recorded in Argentina. More >

  • Open Access

    ARTICLE

    Effect of sugars on the association between cowpea vicilin (7S storage proteins) and fungal cells

    T.L. Rose*, V.M. Gomes*, M. Da Cunha**, K.V.S. Fernandes***, J. Xavier-Filho***

    BIOCELL, Vol.27, No.2, pp. 173-179, 2003, DOI:10.32604/biocell.2003.27.173

    Abstract Vicilins (7S storage proteins) found in various legume seeds have been previously shown to interfere with the germination of spores or conidia of phytopathogenic fungi and inhibit yeast growth and glucose stimulated acidification of the medium by yeast cells. In the present work vicilins from cowpea (Vigna unguiculata) seeds were added to the growth medium of Saccharomyces cerevisiae cells and Fusarium oxysporum conidia. Helix pomatia lectin, wheat germ agglutinin and Ulex europaeus lectin were used to identify differences in the binding of the vicilins to the surface of cells of S. cerevisiae and F. oxysporum treated with this protein. After the growth More >

  • Open Access

    ARTICLE

    Distribution of pectins in the pollen apertures of Oenothera hookeri.velans ster/+ster:

    I.Noher de Halac1,2, I.A. Cismondi2, M.I. Rodriguez-García3, G.Famá

    BIOCELL, Vol.27, No.1, pp. 11-18, 2003, DOI:10.32604/biocell.2003.27.011

    Abstract Cell wall pectins are some of the most complex biopolymers known, and yet their functions remain largely mysterious. The aim of this paper was to deepen the study of the spatial pattern of pectin distribution in the aperture of Oenothera hookeri.velans ster/+ster fertile pollen. We used “in situ” immunocytochemical techniques at electron microscopy, involving monoclonal antibodies JIM5 and JIM7 directed against pectin epitopes in fertile pollen grains of Oenothera hookeri.velans ster/+ster. The same region was also analyzed by classical cytochemistry for polysaccharide detection. Immunogold labelling at the JIM7 epitope showed only in mature pollen labelling mainly located… More >

  • Open Access

    ARTICLE

    Brief Note : Micropropagation of Glandularia perakii Cov. et Schn. (Verbenaceae), a native species with ornamental potential

    Concepción Marino1, María T. Ponce1,*, María E. Videla2, Sonia Fioretti3, Miguel Cirrincione1

    BIOCELL, Vol.27, No.1, pp. 57-60, 2003, DOI:10.32604/biocell.2003.27.057

    Abstract Glandularia perakii is a perennial species with beautiful violet flowers that grows in the stony soil of Mendocine pedemont. A plentiful and prolonged flowering confers it an important ornamental potential. In this paper, a method of propagation of G. perakii from nodal segments is reported. Proliferating microshoot cultures were obtained by placing nodal segment on Murashige and Skoog medium (MS) supplemented with 20 g.L-1 of sucrose without growth regulators. In this medium multiplication rate after 20 days was 7.9. Rooted plants were acclimatized successfully . More >

  • Open Access

    ARTICLE

    Bone and Joints Modelling with Individualized Geometric and Mechanical Properties Derived from Medical Images

    M.C. Ho Ba Tho1

    CMES-Computer Modeling in Engineering & Sciences, Vol.4, No.3&4, pp. 489-496, 2003, DOI:10.3970/cmes.2003.004.489

    Abstract The objective of the paper is to address the methodology developed to model bone and joints with individualised geometric and material properties from medical image data. An atlas of mechanical properties of human bone has been investigated demonstrating individual differences. From these data, predictive relationships have been established between mechanical properties and quantitative data derived from measurements on medical images. Subsequently, geometric and numerical models of bones with individualised geometrical and mechanical properties have been developed from the same source of image data. The advantages of this modelling technique are its ability to study the More >

  • Open Access

    ARTICLE

    Entry Length and Wall Shear Stress in Uniformly Collapsed-Pipe Flow

    M. Thiriet1, S. Naili2, C. Ribreau2

    CMES-Computer Modeling in Engineering & Sciences, Vol.4, No.3&4, pp. 473-488, 2003, DOI:10.3970/cmes.2003.004.473

    Abstract The laminar steady flow of incompressible Newtonian fluid is studied in rigid pipes with cross configuration of a collapsed tube to determine both the entry length and the wall shear stress (WSS). The cross section shapes have been defined from the collapse of an infinitely long elastic tube subjected to an uniform transmural pressure. Five characteristic collapsed configurations, from the unstressed down to the point-contact states, with a finite and infinite curvature radius at the contact point, are investigated, although the wall contact is not necessary observed in veins. Such collapsed shapes induce cross gradient More >

  • Open Access

    ARTICLE

    A Discrete Model for the High Frequency Elastic Wave Examination on Biological Tissue

    Jun Liu1, Mauro Ferrari1

    CMES-Computer Modeling in Engineering & Sciences, Vol.4, No.3&4, pp. 421-430, 2003, DOI:10.3970/cmes.2003.004.421

    Abstract A microstructure-accounting mechanical field theory approach is applied to the problem of reflection from a granular thin layer embedded between two solid substrates to study the direct relationship of the micro-structural parameters and the overall reflection coefficients of the thin layer. The exact solution for plane wave reflection coefficients is derived under the new theoretical framework giving quantitative relations between the macroscopic reflection coefficients and a set of micro structural/physical parameters including particle size and micromoduli. The model was analyzed using averaged material properties of biological tissue for the granular thin layer. It was demonstrated More >

  • Open Access

    ARTICLE

    A Method for Estimating Relative Bone Loads from CT Data with Application to the Radius and the Ulna

    K.J. Fischer1,2, J.A. Bastidas3, H.J. Pfaeffle2, J.D. Towers2

    CMES-Computer Modeling in Engineering & Sciences, Vol.4, No.3&4, pp. 397-404, 2003, DOI:10.3970/cmes.2003.004.397

    Abstract The two bones of the forearm, the radius and the ulna, have been shown to bear different proportions of the overall forearm load at the wrist and the elbow. This biomechanical data suggests load transfer between the bones occurs through the soft tissues of the forearm. Load transfer from radius to ulna through passive soft tissues such as the interosseous ligament (IOL) has been experimentally measured. Ex vivo studies of the forearm, however, cannot account for the effect of internal loads generated by the muscles and, in some cases, external forces acting directly on the… More >

Displaying 10781-10790 on page 1079 of 10856. Per Page