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  • Open Access

    ARTICLE

    Antifungal Activity of Schinifoline Against Candida Albicans in Caenorhabditis Elegans

    Chengjie Shu1, Mengli Zhang1, Weiming Zhang1, Shihong Ma1, Guoqing Chen2 and Baojun Shi1,*

    Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 317-324, 2019, DOI:10.32604/phyton.2019.07766

    Abstract Zanthoxylum schinifolium has been used as spices and traditional medicine in China for hundreds of years. A variety of active substances have been isolated from Zanthoxylum schinifolium using biological and chemical techniques. Among these substances, the effect of schinifoline has gradually attracted much attention. Candida albicans is one of the most common pathogens isolated from the gastrointestinal tract, vagina, and mouth in healthy individuals. In a healthy population, there are various mechanisms in host, such as the microbial flora, the epithelial barriers, and the innate immune system, that can control the presence of Candida albicans.… More >

  • Open Access

    ARTICLE

    The Origin and Identity of the Calyculus in Loranthaceae: Inferred From the Floral Organogenesis of Loranthus tanakae Franch. & Sav.

    Ruozhu Lin1, Bei Cui1,2 and Wenxia Zhao1,*

    Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 285-293, 2019, DOI:10.32604/phyton.2019.07182

    Abstract The flowers of the hemiparasitic family Loranthaceae are always subtended by a rimmed structure known as the calyculus. The origin and identity of the calyculus have been disputed for more than a century. Various hypotheses have been proposed, for example, an outgrowth of the axis, a reduced calyx, and a bracteolar (prophyllar) origin, but controversies remain. To obtain a plausible explanation of the origin of the calyculus, we investigated the flowers of Loranthus tanakae using scanning electron microscopy and light microscopy to observe the entire developmental process of the floral parts. Our results show that… More >

  • Open Access

    ARTICLE

    Morphometric and Biochemical Changes in Agave americana L. Plantlets Induced By Ethyl Methanesulfonate

    S. J. Reyes-Zambrano1,†, M. L. Ramírez-Merchant1,†, C. Arias-Castro2, M. A. Rodríguez-Mendiola2, C. A. Lecona-Guzmán1, V. M. Ruíz-Valdiviezo1, D. González-Mendoza3, F. A. Gutiérrez-Miceli1,*

    Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 277-284, 2019, DOI:10.32604/phyton.2019.06504

    Abstract A. americana L. is a crop with very little genetic variability. In order to evaluate the effect of ethyl methanesulfonate (EMS) to induce variability in in vitro plantlets of A. americana, different explants (meristems, leaves and roots) were evaluated for the production of callus. MS medium supplemented with ANA (2.68 μM) and BAP (2.68 μM) was used. Callus obtained from apical meristem were treated with 15 mM EMS for two hours after which shoot formation was induced using 2,4-D (0.11 μM) and BAP (44 μM). The EMS induced variations in the morphometric and morphological parameters of the plantlets More >

  • Open Access

    ARTICLE

    Biochar Effectively Reduces Ammonia Volatilization From Nitrogen-Applied Soils in Tea and Bamboo Plantations

    Lei Chu, H. M. K. Darshika Hennayake, Haijun Sun*

    Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 261-267, 2019, DOI:10.32604/phyton.2019.07791

    Abstract Intensive practices in forest soils result in dramatic nitrogen (N) losses, particularly ammonia (NH3) volatilization, to adjacent environmental areas. A soil column experiment was conducted to evaluate the effect of bamboo biochar on NH3 volatilization from tea garden and bamboo forest soils. The results showed that biochar amendment effectively reduced NH3 volatilization from tea garden and bamboo forest soil by 79.2% and 75.5%, respectively. The soil pH values increased by 0.53-0.61 units after biochar application. The NH4+-N and total N of both soils were 13.8-29.7% and 34.0-41.9% higher under the biochar treatments than under the control treatment, More >

  • Open Access

    ARTICLE

    Histopathological patterns of ovarian lesions: A study of 161 cases

    Abdulkareem Younis SULEIMAN1, Intisar Salim PITY2, Mohammed R MOHAMMED2, Bashar Abduljabar HASSAWI2

    BIOCELL, Vol.43, No.3, pp. 175-181, 2019, DOI:10.32604/biocell.2019.06884

    Abstract Ovarian lesions are commonly encountered pathologies that cannot be categorized clinicoradiologically. Definite diagnosis is of great importance for therapeutic and prognostic purposes. Histopathology gives accurate diagnosis in most cases. Few cases need supportive tests like immunohistochemistry. Objective: to study the histomorphological diversity of ovarian lesions, their age and location in North of Iraq (Mosul and Duhok). Patients and methods: In the period extended from January 2008 to December 2011, 161 cases of ovarian lesions were collected from pathology departments in Azadi General Hospital “Duhok” and Al-Jamhori Teaching Hospital “Mosul”. Automated tissue processor was used More >

  • Open Access

    ARTICLE

    Detection of ROS and translocation of ERP-57 in apoptotic induced human neuroblastoma (SH-SY5Y) cells

    Atif Kamil1, Mubarak Ali Khan1, Muhammad AAsim2, Nadir Zaman Khan2, Raham Sher Khan1, Muhsin Jamal3, Waqar Ahmad4, Mir Azam Khan4, Fazal Jalil4

    BIOCELL, Vol.43, No.3, pp. 167-174, 2019, DOI:10.32604/biocell.2019.06729

    Abstract Several toxic compounds are known to induce apoptosis in mammalian cell lines. The human neuroblastoma cells (SH-SY5Y) were exposed to the phosphatase inhibiting toxin okadaic acid (OA) or hydrogen peroxide (H2O2) to induce apoptosis as well as generate reactive oxygen species (ROS). Mitoxantrone (MXT) was used as a positive control for apoptosis. The SH-SY5Y cells were transfected with eukaryotic expression plasmid pHyPer-dMito encoding mitochondrial-targeted fluorescent or pHyPer-dCito encoding cytoplasmic-targeted fluorescent sensor for hydrogen peroxide (HyPer). The ERp57, also called GRP58 (Glucose-regulated protein 58), is a stress protein induced in conditions like glucose starvation and More >

  • Open Access

    ARTICLE

    Physiological, Biochemical and Molecular Responses of Barley Seedlings to Aluminum Stress

    Xiaoqin Zhang, Tao Tong, Bin Tian, Yunxia Fang, Jiangjie Pan, Junjun Zheng and Dawei Xue*

    Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 253-260, 2019, DOI:10.32604/phyton.2019.06143

    Abstract Barley (Hordeum vulgare L.) is one of the most Aluminum (Al) sensitive cereal species. In this study, the physiological, biochemical, and molecular response of barley seedlings to Al treatment was examined to gain insight into Al response and tolerance mechanisms. The results showed that superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activity were inhibited to different degrees following Al exposure. The MDA content also significantly increased with increasing Al concentrations. SRAP results indicated significant differences between Al treatments and controls in terms of SRAP profile, and the genomic template stability (GTS) decreased with increasing More >

  • Open Access

    ARTICLE

    Photosynthetic Behaviour and Mineral Nutrition of Tamarix gallica Cultivated Under Aluminum and NaCl Combined Stress

    Dhouha Belhaj Sghaier1, Insaf Bankaji1, Sylvia Pedro2, Isabel Caçador2, Noomene Sleimi1,*

    Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 239-252, 2019, DOI:10.32604/phyton.2019.06887

    Abstract The lack of knowledge of plant tolerance and differential response to aluminum (Al) encouraged many researchers, in the last decade, to elucidate Al toxicity and tolerance mechanisms. The current study reported the impact of Al, a toxic element with negative effects on plant growth and development, in halophytic plant Tamarix gallica. Plants were subjected to different Al concentrations (0, 200, 500 and 800 μM) with or without NaCl (200 mM) supplementation. Growth, photosynthesis and mineral content were assessed. Al stress had a significant decrease on shoots’ biomass production between 19 to 41%, and a little variation… More >

  • Open Access

    ARTICLE

    Cloning and characterization of 66 kDa streptavidin-binding peptides (SBP) of Pisum sativum L. embryo specific to var. Alaska

    Mahmoud MOUSTAFA1,2 , Saad ALAMRI1, Tarek TAHA3, Ali SHATI1, Sulaiman ALRUMMAN1, Mohamed ALKAHTANI1

    BIOCELL, Vol.43, No.3, pp. 155-166, 2019, DOI:10.32604/biocell.2019.06814

    Abstract The aim of the current research was to clone and to characterize the partial 66 kDa streptavidin-binding peptide (SBP) found in the germinated embryos of Pisum sativum L. var. Alaska. The pea (P. sativum var. Alaska) embryos possess prominent 66 kDa SBPs that gradually disappeared after few hours of germination in germinated embryos, but not in the cotyledons. The total RNA was isolated from embryos of P. sativum but could not be isolated from the cotyledons. The partial nucleotides sequences of 66 kDa SBPs of embryonic stalk (P. sativum var. Alaska) were cloned and identified using pMOSBlue vector.… More >

  • Open Access

    REVIEW

    Targeting Glycinebetaine for Abiotic Stress Tolerance in Crop Plants: Physiological Mechanism, Molecular Interaction and Signaling

    Mirza Hasanuzzaman1,*, Aditya Banerjee2, M. H. M. Borhannuddin Bhuyan3,4, Aryadeep Roychoudhury2,*, Jubayer Al Mahmud5 and Masayuki Fujita3

    Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 185-221, 2019, DOI:10.32604/phyton.2019.07559

    Abstract In the era of climate change, abiotic stresses (e.g., salinity, drought, extreme temperature, flooding, metal/metalloid(s), UV radiation, ozone, etc.) are considered as one of the most complex environmental constraints that restricts crop production worldwide. Introduction of stress-tolerant crop cultivars is the most auspicious way of surviving this constraint, and to produce these types of tolerant crops. Several bioengineering mechanisms involved in stress signaling are being adopted in this regard. One example of this kind of manipulation is the osmotic adjustment. The quarternary ammonium compound glycinebetaine (GB), also originally referred to as betaine is a methylated… More >

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