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  • Open Access

    ARTICLE

    Exocytosis determination of SH-SY5Y single cell stimulated by different stimulants on indium tin oxide (ITO) micro-pore electrode

    Hui ZHAO1,2, Fan ZHANG1*, Ling LI1, Pingang HE1, Yuzhi FANG1

    BIOCELL, Vol.37, No.3, pp. 77-83, 2013, DOI:10.32604/biocell.2013.37.077

    Abstract The human neuroblastoma SH-SY5Y cell line has been used as a model to study mechanisms of neurotransmitter release. In order to study the mechanism of SH-SY5Y single cell exocytosis stimulated by different stimulants, including high K+, 3-(1-nitroso-2-pyrrolidinyl) pyridine and nicotine, a type of indium tin oxide (ITO) micro-pore electrode was used to obtain the corresponding amperometric response time. When the cell is stimulated by 0.1 M K+ , almost immediate exocytosis could be detected, due to the rapid depolarization of cell membrane. However, the stimulations with 1 mM nicotine and 3-(1-nitroso-2-pyrrolidinyl) pyridine result in a More >

  • Open Access

    ARTICLE

    Stability parameters in yield and adaptability for rice 25 genotypes of Campeche, Mexico

    Orona Castro F1, J Medina Méndez1, FM Tucuch Cauich1, JM Soto Rocha1, IH Almeyda León2

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 255-261, 2013, DOI:10.32604/phyton.2013.82.255

    Abstract We conducted a comparative study from 2008 to 2011 to study the adaptability and stability of grain yield of upland rice in two regions of Campeche, Mexico. The individual and combined statistical analysis, showed highly significant differences in grain yield of different genotypes in both regions. The experimental error variance was uniform, both effects of repetition/location and variety/location were highly significant. The results of analysis for grain yield stability parameters showed highly significant effects for 12 treatments, and significant for one. Eighteen genotypes showed a significant variation in the effects of deviation from the regression More >

  • Open Access

    ARTICLE

    Effects of plow pan on SPAD value and chloroplast ultrastructure in leaves of spring maize

    Li G, FT Yang, XL Jiang, FX Chen, TH Cao, LC Wang

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 243-247, 2013, DOI:10.32604/phyton.2013.82.243

    Abstract The aim of this study was to evaluate the impact of the plow pan on the production of spring corn. We evaluated SPAD values and chloroplast ultrastructure in premature ear leaves of spring maize at the grain-filling stage. The maize plants were grown in simulated plow pan or simulated subsoiling treatments. Plants in the simulated plow pan treatment showed irreversible damage to chloroplasts, including changes in chloroplast shape, disintegration and rupture of chloroplast membranes, and blurriness and cloudiness of grana lamellae. The chlorophyll content decreased, which is a typical characteristic of senescence. Subsoiling cultivation practices More >

  • Open Access

    ARTICLE

    First report of Tobacco mosaic virus (TMV) and Poinsettia mosaic virus (PnMV) in poinsettia (Euphorbia pulcherrima Willd. Ex Klotzch) in Mexico

    Ocampo Ocampo T1, DL Ochoa Martínez1, S Ramírez Rojas2, G Valdovinos Ponce1, C Nava Díaz1

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 235-241, 2013, DOI:10.32604/phyton.2013.82.235

    Abstract The Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), in Zacatepec Morelos, started a genetic improvement program of wild and semi-cultivated poinsettia plants ("nochebuena de sol"). It is important to generate a phytosanitary database that allows the release of new varieties and hybrids that provide tolerance to the major pathogens reported in cultivated poinsettia plants. The phytosanitary status of the “nochebuena de sol” plants is not available in Mexico, so the objective of this research was to generate preliminary data about viruses associated to these materials. Based on DAS-ELISA and RT-PCR results, we reported More >

  • Open Access

    ARTICLE

    Genetic variability of nanche in Tabasco, Mexico, determined with RAPDs

    Martínez ME1, JM Lesher G2, G Castañón N2, E de la Cruz L1, C Zapata H2

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 209-214, 2013, DOI:10.32604/phyton.2013.82.209

    Abstract Nanche (Byrsonima crassifolia (L.) H.B.K.) grows in the southeastern region of the Mexican republic under both wild and semi-cultivated forms. Despite the importance of the fruit of this tree, only one paper at the molecular level has been published in Mexico, and possibly in America, to date. For this reason, the purpose of this research was to study the genetic variability of nanche trees that grow under both wild and semi-cultivated conditions in the state of Tabasco, Mexico. Molecular determinations were carried out in the Laboratorio de Genómica of the División Académica de Ciencias Biológicas (DACBiológicas),… More >

  • Open Access

    ARTICLE

    Ecological indicators of the weed community in the cultivation of mango Mangifera indica L. in Campeche State, Mexico

    Tucuch-Cauich FM1, F Orona-Castro1, IH Almeyda-León2, LA Aguirre-Uribe3

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 145-149, 2013, DOI:10.32604/phyton.2013.82.145

    Abstract Ango is the main species of fruit in the state of Campeche, Mexico, due to the economic benefits generated because of its production and marketing. Among the biotic problems that affect this crop in the region are weeds, which involve a heavy economic investment for their management. Under this scenario, it is important the development of ecological weed studies to generate information that help us to design strategies for managing the problem. During January to June 2010 a floristic study was developed on the population of weeds that compete with mango in the locations of More >

  • Open Access

    ARTICLE

    Protective eect of arbuscular mycorrhizal fungi on plants of tomato (Solanum lycopersicum) exposed to Cr(VI)

    Carreón-Abud Y, MA Beltrán-Nambo, M Martínez Trujillo

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 127-134, 2013, DOI:10.32604/phyton.2013.82.127

    Abstract Chromium (Cr) is a highly toxic non-essential metal for microorganisms and plants; due to its frequent industrial use, it has become a pollutant for different agroecosystems. Arbuscular mycorrhizal fungi (AMF) are associated with most angiosperms and provide them with beneficial effects on the absorption of some nutrients. Moreover, it has been reported that in some cases the AMF can confer greater tolerance to plants in the presence of heavy metals. The tomato (Solanum lycorpersicum) is an important crop in Mexico. In most cases, it grows under applied irrigation water from reservoirs which include domestic and industrial… More >

  • Open Access

    ARTICLE

    Fungi degrading metsulfuron methyl in agricultural soils of Argentina

    Vázquez MB1, MV Bianchinotti1,2

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 113-118, 2013, DOI:10.32604/phyton.2013.82.113

    Abstract Strains of Mucor, Penicillium and Trichoderma, able to use metsulfuron-methyl (MM) as a sole carbon and energy source, were isolated from agricultural soils in Argentina. This is the first time this ability is recorded in the Mucor or Trichoderma species. When the isolated strains were compared in relation to their capacity to use the herbicide, those of Trichoderma showed the best results. Because of this, they were selected for further assays. The effect of metsulfuron on growth in liquid media was tested. Also, the ability of conidia to germinate in water agar medium containing the target herbicide was More >

  • Open Access

    ARTICLE

    Comparative effectiveness of two nitrogen sources for corn fertilization

    Copperi MZ1, ME M,olesi2, MA Cantamutto2, MM Ron2

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 99-106, 2013, DOI:10.32604/phyton.2013.82.099

    Abstract Corn has not only a high growth potential and a great capacity of biological response to suitable crop management but also may contribute to a better match of crops with environmental offer in Argentina. Nitrogen (N) fertilization must make provision for eventual N losses, and fertilization strategies must be designed so as to minimize their global incidence. The different quick-release N sources show similar efficiencies when they are incorporated, but in surface applications, sources that contain little or no amidic N have a better performance than urea (U). The objective of this paper was to… More >

  • Open Access

    ARTICLE

    Accumulation of silver nanoparticles and its effect on the antioxidant capacity in Allium cepa L.

    Juárez-Maldonado1 A, JL Rosales-Velázquez2, H Ortega-Ortiz3, M Cabrera-De-la-Fuente1, H Ramírez1, A Benavides-Mendoza1

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 91-97, 2013, DOI:10.32604/phyton.2013.82.091

    Abstract Nanotechnology is currently an important worldwide study field because it provides control on matter at a nanometric scale. In food and agricultural fields, the applications of this technology are in early stages. Onion is one of the main vegetables grown in the world. Onion is a low calorie food which contains a large amount of flavonoids. In this work, silver nitrate application in onion was evaluated. An onion crop was developed at the University Antonio Narro in Saltillo, Mexico. Two methods of silver nitrate application were used (a nutritive solution: 0, 20, 40 and 80… More >

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