Denis Arsenijevic¹, Noemi Hernadfalvi¹, Claudia von Meyenburg¹, Brigitte Onteniente², Denis Richard³, Wolfgang Langhans¹

European Cytokine Network, Vol.18, No.2, pp. 93-101, 2007, DOI:10.1684/ecn.2007.0091

Abstract Since the redox state regulator glutathione (GSH), which influences lipopolysaccharide (LPS) anorexia, may be controlled by cytokines, we studied the roles of tumour necrosis factor-alpha (TNFα) and nerve growth factor (NGF) in the GSH response to intraperitoneal (ip) LPS injection in mice. Basal NGF and total reduced GSH (trGSH) levels were up-regulated in brain and liver of TNFα-knock-out (KO) mice, and this was associated with attenuated LPS anorexia. The increases in NGF and trGSH presumably contributed to the attenuated anorexia in response to LPS because transgenic mice over-expressing NGF (NGF-tg mice) also had increased trGSH… More >

Noemi Hernadfalvi¹, Wolfgang Langhans¹, Claudia von Meyenburg¹, Brigitte Onteniente², Denis Richard³, Denis Arsenijevic¹

European Cytokine Network, Vol.18, No.2, pp. 86-92, 2007, DOI:10.1684/ecn.2007.0090

Abstract To study the role of the redox state regulator glutathione (GSH) in bacterial lipopolysaccharide (LPS)-induced anorexia we measured total reduced GSH (trGSH) in liver, serum and brain in response to intraperitoneal (ip) lipopolysaccharide (LPS, 4 lg/mouse) injection in LPS-naïve and LPS-pretreated (4 lg/mouse given 3 days earlier) mice. LPS reduced food intake in LPS-naïve mice and LPS pretreatment attenuated this effect. LPS decreased trGSH at 24 hours after injection in LPS-naïve mice but 4 days later trGSH levels were upregulated in brain and liver, and this was associated with a significant attenuation of LPS-induced anorexia. More >

Lorena Salazar^1,2,*, Octavio Aravena^1,2,*, Juan Contreras-Levicoy^1,2, Barbara Pesce^1,2, Diego Catalan^1,2, Roberto Zuniga^1,2, Mirentxu Iruretagoyena^2,3, Alexis M. Kalergis^2,3, Juan C. Aguillón^1,2

European Cytokine Network, Vol.18, No.2, pp. 78-85, 2007, DOI:10.1684/ecn.2007.0089

Abstract Dendritic cells (DCs) are professional, antigen-presenting cells, which induce and regulate T cell reactivity. DCs are crucial in innate and adaptive immune responses, and are also involved in central and peripheral tolerance induction. Tolerance can be mediated by immature and semi-mature DCs expressing low levels of co-stimulator and major histocompatibility complex (MHC) molecules. The aim of this study was to investigate the ability of short-term lipopolysaccharide (LPS) stimulation to modulate the stage of differentiation of bone marrow-derived DCs. For this purpose, DCs obtained from DBA1/lacJ mice were stimulated for four (4hLPS/DCs) or 24 (24hLPS/DCs) hours… More >

Evemie Schutyser^1,2, Yingjun Su², Yingchun Yu², Mieke Gouwy¹, Snjezana Zaja-Milatovic², Jo Van Damme¹, Ann Richmond²

European Cytokine Network, Vol.18, No.2, pp. 59-70, 2007, DOI:10.1684/ecn.2007.0087

Abstract The influence of environmental factors (cytokines, matrix components, serum factors and O₂ level) on expression of receptors for angiogenic versus angiostatic CXC chemokines in human microvascular endothelial cells has not been extensively investigated. Our semi-quantitative RT-PCR analysis demonstrated that TNF-α and IFN-γ repressed CXCR4 mRNA levels in immortalized human microvascular endothelial HMEC-1 cells after 4 h, whereas only TNF-α displayed inhibitory activity in primary human microvascular endothelial cells (HMVEC). CXCR4 mRNA expression was not affected by VEGF, GM-CSF, IL-1β or various basal membrane matrix components, but was significantly up-regulated after serum starvation and/or hypoxic treatment of… More >

Andrzej Torbé, Ryszard Czajka, Agnieszka Kordek, Rafał Rzepka, Sebastian Kwiatkowski, Jacek Rudnicki

European Cytokine Network, Vol.18, No.2, pp. 57-62, 2007, DOI:10.1684/ecn.2007.0092

Abstract Our aim was to compare maternal serum concentrations of interleukin(IL)-1α IL-1β, IL-6 and IL-8 in pregnancies complicated by preterm labor (PTL), with the levels in healthy controls at approximately full-term, and to determine if these assays have any value in the prediction of early-onset neonatal infection or histological chorioamnionitis. The study population consisted of 65 women with new-onset PTL, and 31 healthy controls. Maternal serum concentrations of IL-6 (8.40 versus 3.30 pg/mL; p = 0.002) and IL-1β (2.20 versus 0.50 pg/mL; p = 0.003) were significantly higher in patients with PTL as compared to healthy… More >

Denis Arsenijevic¹, Noemi Hernadfalvi¹, Claudia von Meyenburg¹, Brigitte Onteniente², Denis Richard³, Wolfgang Langhans¹

European Cytokine Network, Vol.18, No.2, pp. 47-55, 2007, DOI:10.1684/ecn.2007.0091

Abstract Since the redox state regulator glutathione (GSH) influences lipopolysaccharide (LPS) anorexia, we studied the roles of tumour necrosis factor-alpha (TNFα) and nerve growth factor (NGF) in the GSH response to intraperitoneal (ip) LPS injection in mice. Basal NGF and GSH levels were up-regulated in brain and liver of TNFα-knock-out (KO) mice, and this was associated with attenuated LPS anorexia. The increases in NGF and GSH presumably contributed to the attenuated anorexia in response to LPS because transgenic mice over-expressing NGF (NGF-tg) also had increased GSH levels and displayed attenuated anorexia compared to the corresponding wild… More >

Noemi Hernadfalvi¹, Wolfgang Langhans¹, Claudia von Meyenburg¹, Brigitte Onteniente², Denis Richard³, Denis Arsenijevic¹

European Cytokine Network, Vol.18, No.2, pp. 39-45, 2007, DOI:10.1684/ecn.2007.0090

Abstract To study the role of the redox state regulator glutathione (GSH) in bacterial lipopolysaccharide (LPS)-induced anorexia we measured GSH in liver, serum and brain in response to intraperitoneal (ip) lipopolysaccharide (LPS, 4lg/mouse) injection in LPS-naïve and LPS-pretreated (4 lg/mouse) mice. LPS reduced food intake in LPS-naïve mice and LPS pretreatment attenuated this effect. LPS reduced total reduced GSH at 24 hours after injection in LPS-naïve mice. On the other hand, LPS pretreatment caused an upregulation of GSH levels in brain and liver, and this was associated with a significant attenuation of LPS-induced anorexia. In addition, More >

Lorena Salazar^1,2,*, Octavio Aravena^1,2,*, Juan Contreras-Levicoy^1,2, Barbara Pesce^1,2, Diego Catalan^1,2, Roberto Zuniga^1,2, Mirentxu Iruretagoyena^2,3, Alexis M. Kalergis^2,3, Juan C. Aguillón^1,2

European Cytokine Network, Vol.18, No.2, pp. 31-38, 2007, DOI:10.1684/ecn.2007.0089

Abstract Dendritic cells (DCs) are professional, antigen-presenting cells, which induce and regulate T cell reactivity. DCs are crucial in innate and adaptive immune responses, and are also involved in central and peripheral tolerance induction. Tolerance can be mediated by immature and semi-mature DCs expressing low levels of co-stimulator and major histocompatibility complex (MHC) molecules. The aim of this study was to investigate the ability of short-term lipopolysaccharide (LPS) stimulation to modulate the stage of differentiation of bone marrow-derived DCs. For this purpose, DCs obtained from DBA1/lacJ mice were stimulated for four (4hLPS/DCs) or 24 (24hLPS/DCs) hours… More >

Evemie Schutyser^1,2, Yingjun Su², Yingchun Yu², Mieke Gouwy¹, Snjezana Zaja-Milatovic², Jo van Damme¹, Ann Richmond²

European Cytokine Network, Vol.18, No.2, pp. 11-22, 2007, DOI:10.1684/ecn.2007.0087

Abstract The influence of environmental factors (cytokines, matrix components, serum factors and O₂ level) on expression of receptors for angiogenic versus angiostatic CXC chemokines in human microvascular endothelial cells has not been extensively investigated. Our semi-quantitative RT-PCR analysis demonstrated that TNF-α and IFN-γ repressed CXCR4 mRNA levels in immortalized human microvascular endothelial HMEC-1 cells after 4 h, whereas only TNF-a displayed inhibitory activity in primary human microvascular endothelial cells (HMVEC). CXCR4 mRNA expression was not affected by VEGF, GM-CSF, IL-1β or various basal membrane matrix components, but was significantly up-regulated after serum starvation and/or hypoxic treatment of… More >

Jesus F. Bermejo-Martin¹, Maria C. Garcia-Arevalo¹, Raul Ortiz De Lejarazu², Julio Ardura³, Jose M. Eiros², Ana Alonso³, Vanesa Matías³, Maria Pino³, David Bernardo¹, Eduardo Arranz¹, Alfredo Blanco-Quiros¹

European Cytokine Network, Vol.18, No.3, pp. 162-167, 2007, DOI:10.1684/ecn.2007.0096

Abstract Profiling of immune mediators in both nasal and plasma samples is a common approach to the study of pathogenesis in respiratory viral infections. Nevertheless, mucosal immunity functions essentially independently from peripheral immunity. In our study, 27 immune mediators were profiled in parallel, in nasopharyngeal aspirates (NPAs) and plasma from 22 < 2 year-old children with a severe respiratory syncytial virus infection involving the lower respiratory tract, using a multiplex assay. NPAs from 22 children with innocent heart murmurs were used as controls. Differences in mediator concentrations between NPAs from patients and controls were assessed using… More >