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  • Open Access

    ARTICLE

    Structural and ultrastructural characterization of zebu (Bos indicus) spermatozoa

    MARIA CAROLINA AQUINO LUQUE, SÔNIA NAIR BÁO

    BIOCELL, Vol.30, No.1, pp. 33-38, 2006, DOI:10.32604/biocell.2006.30.033

    Abstract The ultrastructure of normal, ejaculated spermatozoa of Bos indicus was studied by means of electron microscopy, being evaluated in two principal parts, the head and the tail. The head is flat, oval or paddle-shaped with a square base, which provides a concave recess for the insertion of the tail. The acrosome tightly covers the anterior two thirds of the nucleus. A distinct unilateral acrosomal bulge was observed along the apical edge of the head. The equatorial region demarcates the acrosome from the post-equatorial region that covers the caudal one third of the nucleus. The classical 9+9+2 More >

  • Open Access

    ARTICLE

    Visualization of the ribosomal DNA (45S rDNA) of Indica rice with FISH on some phases of cell cycle and extended DNA fibers

    ZONG-YUN LI1,2*, MEI-LI FU1, FANG-FANG HU1, SHU-FENG HUANG1, YUN-CHUN SONG2

    BIOCELL, Vol.30, No.1, pp. 27-32, 2006, DOI:10.32604/biocell.2006.30.027

    Abstract The ribosomal DNA (45S rDNA) behaviors during the cell cycle were analyzed on interphase nuclei, prophases, metaphases, pachytene chromosomes and extended DNA fibers in rice (Oryza,sativa ssp.indica cv.Guangluai No.4) by using high-resolution fluorescent in situ hybridization (FISH). The results show that 45S rDNA is located at the ends of short arms of chromosomes 9 and 10. But the signals are much more intense on chromosome 9 than on chromosome 10 in metaphase. Pachytene chromosome has rDNA signal arrays on chromosome 9. Different phases are described and discussed. These results indicate that the activity of rDNA at individual More >

  • Open Access

    ARTICLE

    Genomic cloning and characterization of a PPA gene encoding a mannose-binding lectin from Pinellia pedatisecta

    JUAN LIN1, XUANWEI ZHOU2, JIONG FEI2, ZHIHUA LIAO1, WANG JIN1, XIAOFEN SUN1,*, KEXUAN TANG1,2,*

    BIOCELL, Vol.30, No.1, pp. 15-25, 2006, DOI:10.32604/biocell.2006.30.015

    Abstract A gene encoding a mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was isolated from leaves of Pinellia pedatisecta using genomic walker technology. The ppa contained an 1140-bp 5’-upstream region, a 771-bp open reading frame (ORF) and an 829-bp 3’-downstream region. The ORF encoded a precursor polypeptide of 256 amino acid residues with a 24-amino acid signal peptide. There were one putative TATA box and six possible CAAT boxes lying in the 5’-upstream region of ppa. The ppa showed significant similarity at the nucleic acid level with genes encoding mannose-binding lectins from other Araceae species such as Pinellia ternata, Arisaema heterophyllum, Colocasia esculenta More >

  • Open Access

    ARTICLE

    Biological efficiency of organic. biological and chemical products against Alternaria dauci Kühn and its effects on carrot crop

    Hernández-Castillo1 FD, Adalberto Aguirre-Aguirre1, RH Lira-Saldivar2, E Guerrero-Rodríguez1, G Gallegos-Morales1

    Phyton-International Journal of Experimental Botany, Vol.75, pp. 91-101, 2006, DOI:10.32604/phyton.2006.75.091

    Abstract The biological efficiency of five stocks of Bacillus subtilis, of the mixture of the bioproducts quitosan and Larrea tridentata extract, and five synthetic fungicides were evaluated against the fungus Alternaria dauci Kühn in laboratory and greenhouse experiments. The effect of these compounds on growth and yield of carrot was also determined. Stocks of the bacteria Bacillus (B3, B9, B15 and a combination of these) and the mixture quitosan- Larrea stimulated carrot development because it reached greater width, length and weight in comparison to the control and the other treatments. Stock B3 was the best in promoting carrot growth More >

  • Open Access

    ARTICLE

    Hydraulic lift in and between vegetation patches in arid Argentina

    Bonvissuto GL1, CA Busso2,*

    Phyton-International Journal of Experimental Botany, Vol.75, pp. 55-70, 2006, DOI:10.32604/phyton.2006.75.055

    Abstract This study was conducted in the Monte Austral Neuquino (MAN), Provincia de Neuquén, Argentina (39°20’ S; 69°19’ W). Vegetation is distributed at the site as vegetation patches. There are two contrasting sites on the soil of these patches: location 1, under the canopy of major perennial grasses and shrubs, and location 2, which includes the vegetation uncovered interspaces between the patches. The hypothesis of this work was that hydraulic lift is greater under than between the vegetation patches. Thermocouple psychrometers were placed under the shrubs and in the interspaces in each of two vegetation patches,… More >

  • Open Access

    ARTICLE

    A Strain Energy Density Rate Approach to the BEM Analysis of Creep Fracture Problems

    C.P. Providakis1

    Structural Durability & Health Monitoring, Vol.2, No.4, pp. 249-254, 2006, DOI:10.3970/sdhm.2006.002.249

    Abstract This paper explores the concept of strain energy density rate in relation to the crack initiation in fracture analysis problems arising in creeping cracked structural components. The analysis of the components is performed by using the boundary element methodology in association with the employment of singular boundary elements for the modeling of the crack tip region. The deformation of the material is assumed to be described by an elastic power law creep model. The strain energy density rate theory is applied to determine the direction of the crack initiation for a center cracked plate in More >

  • Open Access

    ARTICLE

    An Alternative BEM for Fracture Mechanics

    G. Davì1, A. Milazzo1

    Structural Durability & Health Monitoring, Vol.2, No.3, pp. 177-182, 2006, DOI:10.3970/sdhm.2006.002.177

    Abstract An alternative single domain boundary element formulation and its numerical implementation are presented for the analysis of two-dimensional cracked bodies. The problem is formulated employing the classical displacement boundary integral representation and a novel integral equation based on the stress or Airy's function. This integral equation written on the crack provides the relations needed to determine the problem solution in the framework of linear elastic fracture mechanics. Results are presented for typical problems in terms of stress intensity factors and they show the accuracy and efficiency of the approach. More >

  • Open Access

    ARTICLE

    From Damage to Crack: A B.E. Approach

    V. Mallardo, C. Alessandri1

    Structural Durability & Health Monitoring, Vol.2, No.3, pp. 165-176, 2006, DOI:10.3970/sdhm.2006.002.165

    Abstract The formation of cracks and their propagation in brittle materials has been intensively studied in the last years. The main difficulty is related to the theoretical and numerical possibility to follow the development of regions of highly localised strains. The nonlinear phenomenon is physically different from the one which occurs in ductile materials: it starts with a narrow fracture process zone containing a large number of distributed microcracks which could lead to the formation of macrocracks and eventually to rupture. In the present paper, a simple nonlocal damage model is coupled to the crack analysis More >

  • Open Access

    ARTICLE

    Aircraft Structural Integrity Assessment through Computational Intelligence Techniques

    RamanaM. Pidaparti1

    Structural Durability & Health Monitoring, Vol.2, No.3, pp. 131-148, 2006, DOI:10.3970/sdhm.2006.002.131

    Abstract This paper provides an overview of the computational intelligence methods developed for the structural integrity assessment of aging aircraft structures. Computational intelligence techniques reviewed include artificial neural networks, inverse neural network mapping, wavelet based image processing methods, genetic algorithms, spectral element methods, and particle swarm optimization. Multi-site damage, corrosion, and corrosion-fatigue damage in aging aircraft is specifically discussed. Results obtained from selected computational intelligence methods are presented and compared to the existing alternate solutions and experimental data. The results presented illustrate the applicability of computational intelligence methods for assessing the structural integrity of aging aircraft More >

  • Open Access

    ARTICLE

    Can the Conventional High-Cycle Multiaxial Fatigue Criteria Be Re-Interpreted in Terms of the Theory of Critical Distances?

    L. Susmel1,2, D. Taylor2

    Structural Durability & Health Monitoring, Vol.2, No.2, pp. 91-108, 2006, DOI:10.3970/sdhm.2006.002.091

    Abstract This paper reports on an attempt to systematically re-interpret the conventional multiaxial fatigue criteria in terms of the Theory of Critical Distances: in the present study the criteria proposed by Crossland, Dang Van, Papadopoulos, Matake, McDiarmid, respectively, and the so-called Modified W\"{o}hler Curve Method were considered. The procedure devised to re-interpret the above methods in terms of the Theory of Critical Distances was based on the following two assumptions: (i) the critical distance is a material constant to be determined under fully-reversed uniaxial fatigue loading; (ii) the presence of non-zero mean stresses as well as… More >

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