Benjamin Piura^1,4, Liat Medina^2,4, Alex Rabinovich^1,4, Victor Dyomin^3,4, Mahmoud Huleihel^2,4

European Cytokine Network, Vol.24, No.3, pp. 122-129, 2013, DOI:10.1684/ecn.2013.0342

Abstract Background. Thalidomide inhibits TNF-α production in lipopolysaccharide-stimulated monocytes. The aim of this study was to evaluate the effect of thalidomide on TNF-α, IL-6 and MMP secretion in epithelial ovarian carcinoma cells. Materials and methods. SKOV-3 cells and primary epithelial ovarian carcinoma cells were cultured in the presence of various concentrations of thalidomide. Cell proliferation was examined by MTT proliferation assay. TNF-α and IL-6 levels were determined in the supernatants of the cell cultures by ELISA, and MMP activity was examined by gelatin zymography. Results. Thalidomide did not significantly affect the proliferation and growth of SKOV-3… More >

Harika Atmaca¹, Gurbuz Gorumlu², Burcak Karaca², Mustafa Degirmenci², Didem Tunali², Yalcin Cirak², Duygu Unuvar Purcu¹, Selim Uzunoglu¹, Bulent Karabulut², Ulus A. Sanli², Ruchan Uslu²

European Cytokine Network, Vol.20, No.3, pp. 121-130, 2009, DOI:10.1684/ecn.2009.0159

Abstract In the present study, we aimed to evaluate the possible synergistic, cytotoxic effects of combination treatment of gossypol and zoledronic acid, in human ovarian cancer cell lines, OVCAR-3 and MDAH-2774, and to elucidate the role of this novel combination treatment on angiogenesis-related molecules in ovarian cancer. The XTT cell viability assay was used for showing cytotoxicity. Both DNA fragmentation by ELISA assay and caspase 3/7 activity measurement were used for demonstrating apoptosis. To elucidate the angiogenic molecules affected by combination treatment, mRNA levels of angiogenic molecules were measured using the Human Angiogenesis RT² Profiler^TM PCR Array… More >

T.H. Satoh², T.A. Surmacz³, O. Nyormoi⁴, C.M. Whitacre¹

BIOCELL, Vol.27, No.1, pp. 47-55, 2003, DOI:10.32604/biocell.2003.27.047

Abstract This study shows a strong association between cell attachment to substratum and activation of β1-integrin-signaling with resistance to the camptothecin derivative topotecan (TPT) in breast cancer cells. We propose a mechanistic-driven approach to sensitize the cells to camptothecins. ZR-75-1 anchoragedependent breast cancer cell line, its derivative 9D3S suspension cells (9D3S-S), and 9D3S cells attached to fibronectin-coated plates (9D3S-A) were treated with TPT (1 µM) or CPT-11 (40 µM) for 48 h. Programmed cell death (PCD), as shown by poly(ADP-ribose) polymerase (PARP), pro-caspase-3 and pro-caspase-9 cleavage, was observed in 9D3S-S cells but not in ZR-75-1 or More >