JUNYING XU^1,#, PING LI^1,#, GE WANG², DAQIANG YE^1,*, XIUFU TANG^3,*

BIOCELL, Vol.48, No.2, pp. 283-291, 2024, DOI:10.32604/biocell.2023.045429 - 23 February 2024

Abstract Background: α7 nicotinic acetylcholine receptor (α7nAChR) has been demonstrated to be involved in numerous of inflammatory diseases. Cell pyroptosis is a kind of cell death accompanied by inflammation. Objectives: The objective of this work is to explore the function of α7nAChR on cell pyroptosis in cervical cancer cells. Methods: Immunoblotting, quantitative polymerase chain reaction, and enzyme-linked immunosorbent assay were employed to examine the function of α7nAChR on cell pyroptosis and metabolic changes. Results: Herein, we found that α7nAChR inhibition led to cell pyroptosis in HeLa and SiHa of cervical cancer cells, which was attributed to the… More >

YANG XU^1,#, HONGYUN LI^1,#, GE YOU^2,*

BIOCELL, Vol.48, No.2, pp. 229-237, 2024, DOI:10.32604/biocell.2023.045030 - 23 February 2024

Abstract Background: Colorectal cancer (CRC) constitutes the leading cause of death worldwide. Chemoresistance and tumor immune evasion are critical contributors to therapeutic failure in cancer patients. CKLF-like MARVEL transmembrane domain-containing 6 (CMTM6) is aberrantly expressed in various cancers and can regulate tumor immunity. However, its role in chemoresistance and tumor immunity of CRC is not well understood. Methods: Online bioinformatics tools were used to analyze expression and prognosis of CMTM6 in CRC patients. CRC cells were transfected with si-CMTM6. Subsequently, the effects on CRC cell viability and chemoresistance were investigated by CCK-8 assay and flow cytometer.… More > Graphic Abstract

QI XIA¹, XING CHEN², QINGHUA MA³, XIANXIU WEN^2,*

BIOCELL, Vol.48, No.2, pp. 217-228, 2024, DOI:10.32604/biocell.2023.027414 - 23 February 2024

Abstract Background: Breast cancer (BC) is the most common cancer and the leading cause of cancer death in women. Immune features play an important role in improving the prognosis prediction of BC. However, while previous immune signatures consisted mainly of immune genes, immune cell-based signatures have been rarely reported. Methods: In this study, we report that a novel immune cell signature is effective in improving prognostic prediction by combining M2 macrophages. We identified 17 differentially infiltrating immune cells between cancer and normal groups. Prognostic features of the four immune cells identified by LASSO COX analysis showed… More >

DONGQIN WANG^1,4, YAN SHI¹, ZHIQIANG WANG⁴, JING ZHANG², LUYAO WANG², HONGYU MA³, SHUHUA SHI², XIAOFU LIAN², HUA HUANG⁴, XIAOJING WANG^1,*, CHAOQUN LIAN^4,*

BIOCELL, Vol.48, No.1, pp. 111-122, 2024, DOI:10.32604/biocell.2023.046903 - 30 January 2024

Abstract Introduction: Among all malignant tumors of the digestive system, pancreatic carcinoma exhibits the highest mortality rate. Currently, prevention and effective treatment are urgent issues that need to be addressed. Methods: The study focused on meiotic nuclear divisions 1 (MND1), integrating data from the Gene Expression Profiling Interactive Analysis (GEPIA) database with prognostic survival analysis. Simultaneously, experiments at cellular level were employed to demonstrate the effect of MND1 on the proliferation and migration of PC. The small-molecule inhibitor of MND1 was used to suppress the migration of PC cells by knocking down MND1 using small interfering… More >

CHONG SHEN, JIAJUN YAN^*, YU REN, ZHIRONG ZHU, XIAOLONG ZHANG, SHUIXIANG TAO

BIOCELL, Vol.48, No.1, pp. 97-109, 2024, DOI:10.32604/biocell.2023.045303 - 30 January 2024

Abstract Introduction: Bladder cancer (BC) has a high incidence and mortality rate worldwide. Suppressor anaphase-promoting complex domain containing 2 (SAPCDC2) is over-expressed in a variety of tumors. Objectives: This study investigated the effects of SAPCD2 knockdown on BC cells. Methods: T24 and UMUC3 cell models and the xenografted BC tumor model with SAPCD2 knockdown were established to observe the malignant phenotype of BC cells by cell counting kit-8 assay, colony formation test, wound healing, and Transwell assay, mRNA and proteins expressions were measured with quantitative real-time polymerase chain reaction, western blotting, and tissue immunohistochemistry. Lithium chloride agonist… More > Graphic Abstract

BEHROOZ JOHARI^1,2,#,*, MILAD PARVINZAD LEILAN^1,#, MAHMOUD GHARBAVI³, YOUSEF MORTAZAVI¹, ALI SHARAFI², HAMED REZAEEJAM⁴

Oncology Research, Vol.32, No.2, pp. 309-323, 2024, DOI:10.32604/or.2023.043576 - 28 December 2023

Abstract The Myc gene is the essential oncogene in triple-negative breast cancer (TNBC). This study investigates the synergistic effects of combining Myc decoy oligodeoxynucleotides-encapsulated niosomes-selenium hybrid nanocarriers with X-irradiation exposure on the MDA-MB-468 cell line. Decoy and scramble ODNs for Myc transcription factor were designed and synthesized based on promoter sequences of the Bcl2 gene. The nanocarriers were synthesized by loading Myc ODNs and selenium into chitosan (Chi-Se-DEC), which was then encapsulated in niosome-nanocarriers (NISM@Chi-Se-DEC). FT-IR, DLS, FESEM, and hemolysis tests were applied to confirm its characterization and physicochemical properties. Moreover, cellular uptake, cellular toxicity, apoptosis, cell More > Graphic Abstract

ZHI ZHANG^1,#, XIAOSONG LI^1,2,#, YING ZHANG^1,2,#, HAO ZHU^1,2, ZHENGUO QIAO³, YANG LU⁴, XIUWEI MI⁴, HUIHUA CAO⁵, GENHAI SHEN^1,*, SONGBING HE^4,*

Oncology Research, Vol.32, No.2, pp. 353-360, 2024, DOI:10.32604/or.2023.042986 - 28 December 2023

Abstract Colorectal cancer (CRC) stands among the top prevalent cancers worldwide and holds a prominent position as a major contributor to cancer-related mortality globally. Absent in melanoma 2 (AIM2), a constituent of the interferon-inducible hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats protein family, contributes to both cancer progression and inflammasome activation. Despite this understanding, the precise biological functions and molecular mechanisms governed by AIM2 in CRC remain elusive. Consequently, this study endeavors to assess AIM2’s expression levels, explore its potential antitumor effects, elucidate associated cancer-related processes, and decipher the underlying signaling pathways in CRC. More > Graphic Abstract

ROGHAYEH GHORBANI¹, MAHMOUD GHARBAVI², ALI SHARAFI^3,4, ELHAM RISMANI⁵, HAMED REZAEEJAM⁶, YOUSEF MORTAZAVI^1,*, BEHROOZ JOHARI^3,*

Oncology Research, Vol.32, No.1, pp. 101-125, 2024, DOI:10.32604/or.2023.044741 - 15 November 2023

Abstract In the present study, we investigated the synergistic effects of targeted methotrexate-selenium nanostructure containing Myc decoy oligodeoxynucleotides along with X-irradiation exposure as a combination therapy on LNCaP prostate cancer cells. Myc decoy ODNs were designed based on the promoter of Bcl-2 gene and analyzed by molecular docking and molecular dynamics assays. ODNs were loaded on the synthesized Se@BSA@Chi-MTX nanostructure. The physicochemical characteristics of nanostructures were determined by FTIR, DLS, UV-vis, TEM, EDX, in vitro release, and hemolysis tests. Subsequently, the cytotoxicity properties of them with and without X-irradiation were investigated by uptake, MTT, cell cycle, apoptosis, and… More > Graphic Abstract

HEE JU SONG, TAEHEE KIM, HAN NA CHOI, SOO JIN KIM, SANG DO LEE^*

Oncology Research, Vol.32, No.1, pp. 151-161, 2024, DOI:10.32604/or.2023.030690 - 15 November 2023

Abstract Lung cancer has the highest mortality rate among all cancers, in part because it readily metastasizes. The tumor microenvironment, comprising blood vessels, fibroblasts, immune cells, and macrophages [including tumor-associated macrophages (TAMs)], is closely related to cancer cell growth, migration, and invasion. TAMs secrete several cytokines, including interleukin (IL)-1β, which participate in cancer migration and invasion. p21-activated kinase 1 (PAK1), an important signaling molecule, induces cell migration and invasion in several carcinomas. Tonicity-responsive enhancer-binding protein (TonEBP) is also known to participate in cancer cell growth, migration, and invasion. However, the mechanisms by which it increases lung… More > Graphic Abstract

WEILING ZHANG^1,2, YONG LI², CAN ZHANG², QING HAN², YU ZHANG², AIQIN HE², WEIPEI ZHU^1,*

BIOCELL, Vol.47, No.12, pp. 2697-2708, 2023, DOI:10.32604/biocell.2023.044314 - 27 December 2023

Abstract Background: This study aimed to explore the expression, function, and molecular mechanism of ANXA8, the gene for annexin 8, in cervical cancer. Methods: The gene expression of the ANX family members in cervical cancer tissues was classified via The Cancer Genome Atlas (TCGA) database. The expression of ANXA8 in paracancerous tissues, cervical cancer tissues, and cell lines was identified by fluorescence quantitative polymerase chain reaction (PCR) and immunohistochemistry. The effects of ANXA8 knockdown on the cellular growth and cell invasion of cervical cancer were examined by MTT, clone-formation assay, scratch test, and Transwell assay. The effect of ANXA8 knockdown… More >