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  • Open Access


    Isolation and molecular characterization of a cax gene from Capsella bursa-pastoris


    BIOCELL, Vol.32, No.3, pp. 229-235, 2008, DOI:10.32604/biocell.2008.32.229

    Abstract A new cation exchangers (CAXs) gene was cloned and characterized from Capsella bursapastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA sequence of cax from C. bursa-pastoris (designated as Cbcax51) was 1754 bp containing a 1398 bp open reading frame encoding a polypeptide of 466 amino-acid residues with a calculated molecular mass of 50.5 kDa and an isoelectric point of 5.69. The predicted CbCAX51 contained an IMP dehydrogenase/GMP reductase domain, two Na+/Ca2+ exchanger protein domains. Comparative and bioinformatics analyses revealed that CbCAX51 showed extensive homology with CAX from other plant species. The expression analysis by different treatments indicated… More >

  • Open Access


    Molecular biology techniques as new alternatives for medicinal plant identification

    Villa-Hernández JM1, B García-Ocón2, E del C Sierra-Palacios2, C Pelayo-Zaldivar3, F Díaz de León-Sánchez1, LJ Pérez-Flores1, JA Mendoza-Espinoza2

    Phyton-International Journal of Experimental Botany, Vol.87, pp. 72-78, 2018, DOI:10.32604/phyton.2018.87.072

    Abstract Mexico bears witness to a long-standing tradition in the use of plants for medicinal purposes; nevertheless, standardized methodological approaches for the proper identification of these are still needed. The problem is especially noticeable during the validation of ingredients in herbal preparations, as so many of them are sold as powders. It is for these reasons that the present study aims to compare the results of classic taxonomical assessments with those obtained using more modern molecular techniques (e.g. PCR-amplified 18S ribosomal RNA gene analysis) in one ethnobotanical case-study carried out in the “Desierto de los Leones” National Park in Mexico City,… More >

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