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  • Open Access

    ARTICLE

    Experimental Characterization of MCF-10A Normal Cells Using AFM: Comparison with MCF-7 Cancer Cells

    Moharam Habibnejad Korayem1,*, Zahra Rastegar2

    Molecular & Cellular Biomechanics, Vol.16, No.2, pp. 109-122, 2019, DOI:10.32604/mcb.2019.04706

    Abstract The mechanical properties of single cells have been recently identified as the basis of an emerging approach in medical applications since they are closely related to the biological processes of cells and human health conditions. The problem in hand is how to measure mechanical properties in order to obtain them more accurately and applicably. Some of the cell’s properties such as elasticity module and adhesion have been measured before using various methods; nevertheless, comprehensive tests for two healthy and cancerous cells have not been performed simultaneously. As a Nanoscale device, AFM has been used for… More >

  • Open Access

    ARTICLE

    Topological Characterization of Book Graph and Stacked Book Graph

    Raghisa Khalid1, Nazeran Idrees1,*, Muhammad Jawwad Saif2

    CMC-Computers, Materials & Continua, Vol.60, No.1, pp. 41-54, 2019, DOI:10.32604/cmc.2019.06554

    Abstract Degree based topological indices are being widely used in computer-aided modeling, structural activity relations, and drug designing to predict the underlying topological properties of networks and graphs. In this work, we compute the certain important degree based topological indices like Randic index, sum connectivity index, ABC index, ABC4 index, GA index and GA5 index of Book graph Bn and Stacked book graph Bm,n. The results are analyzed by using edge partition, and the general formulas are derived for the above-mentioned families of graphs. More >

  • Open Access

    ARTICLE

    A New Model for the Characterization of Frozen Soil and Related Latent Heat Effects for the Improvement of Ground Freezing Techniques and Its Experimental Verification

    Daoming Shen1, Hua Si1,*, Jinhong Xia1, Shunqun Li2

    FDMP-Fluid Dynamics & Materials Processing, Vol.15, No.1, pp. 63-76, 2019, DOI:10.32604/fdmp.2019.04799

    Abstract The correct determination of thermal parameters, such as thermal conductivity and specific heat of soil during freezing, is the most important and basic problem for the construction of an appropriate freezing method. In this study, a calculation model of three stages of soil temperature was established. At the unfrozen and frozen stages, the specific temperatures of dry soil, water, and ice are known. According to the principle of superposition, a calculation model of unfrozen and frozen soils can be established. Informed by a laboratory experiment, the latent heat of the adjacent zone was calculated for More >

  • Open Access

    ARTICLE

    Characterization of new anti-IL-6 antibodies revealed high potency candidates for intracellular cytokine detection and specific targeting of IL-6 receptor binding sites

    Karinna Chouman1, Birgit Korioth-Schmitz1, Markus Sack2, Jörn Engelbert Schmitz1, Anh Tuan Pham1, Rainer Fischer2,4, Stefan Barth5, Torsten Klockenbring1, Rolf Fendel1,6

    European Cytokine Network, Vol.29, No.2, pp. 59-72, 2018, DOI:10.1684/ecn.2018.0409

    Abstract Interleukin-6 (IL-6) expression and secretion, induced by inflammatory processes, stimulate the acute phase response cascade. The overexpression of IL-6 contributes to a variety of inflammatory diseases, e.g. rheumatoid arthritis, Castleman’s disease, multiple myeloma, and prostate cancer. Screening for high amounts of IL-6 in the patients’ blood serum can be crucial for an adequate treatment. In this study, five novel murine monoclonal antibodies (mAbs) reactive to human IL-6 were generated. The mAbs were characterized for potential diagnostic purposes and recombinant antibodies were derived thereof. Initial epitope mapping using a combination of blocking experiments and Hyper-IL-6, a fusion… More >

  • Open Access

    ARTICLE

    Morphological diversity of wild and semi-wild chili populations of Tabasco and the north of Chiapas States, Mexico

    Gálvez Muñoz YA1, E Martínez Moreno2, S Ramírez Vera2, L Latournerie Moreno3, JM Lesher Gordillo1, G Castañón Nájera1*

    Phyton-International Journal of Experimental Botany, Vol.87, pp. 60-67, 2018, DOI:10.32604/phyton.2018.87.060

    Abstract The research was conducted with the aim to identify the variability in situ of wild and semi-wild morphotypes of Capsicum spp. that were found growing in different places of Tabasco and the north of Chiapas States. Morphotypes included “Amashito” (five types), “Pico de paloma” (two types), “Garbanzo”, “Ojo de sapo”, “Ojo de cangrejo”, “Colmillo de lagarto” and “Corazón de pollo”. Such characterization is important because there is an extensive variability of forms cultivated in the country, resulting from a wide range of agroecological diversity as well as diverse forms, colours, flavors and sizes that constitute a valuable… More >

  • Open Access

    ARTICLE

    Characterization of multidrug-resistant Klebsiella pneumoniae isolated from the Chinese cobra Naja atra in a Beijing suburb

    Haifeng WANG1, 2, 3 , Hongxuan HE2

    BIOCELL, Vol.42, No.2, pp. 47-54, 2018, DOI:10.32604/biocell.2018.07006

    Abstract The emergence and spread of antibiotic resistance genes among Bacteria are a serious threat to global health. Their occurrence in animals which are in contact with humans is also important. The Chinese cobra (Naja atra, Elapidae), though a highly venomous species, is appreciated as food and as a source of materials used in traditional Chinese medicine. We are here reporting the isolation of multidrug-resistant Klebsiella pneumoniae (Enterobacteriaceae) from the lung of Naja atra, obtained from a snake farm in a Beijing suburb. Our study analyzed, using gene sequencing, the occurrence of antibiotic resistance genes (ARGs) in… More >

  • Open Access

    ARTICLE

    Preparation and Characterization of Alkyd Resins Based on Citrullus colocynthis Seed Oil

    Hassen Mohamed Sbihi1,*, Hamid Shaikh2, Lahssen El Blidi2, Imed Arbi Nehdi1, Ubair Abdus Samad2, Moufida Romdhani-Younes3, Saud Ibrahim Al-Resayes1

    Journal of Renewable Materials, Vol.6, No.6, pp. 651-661, 2018, DOI:10.7569/JRM.2018.634105

    Abstract In this study, different samples of alkyd resins based on Citrullus colocynthis seed oil (CCSO)—which has a high content of linoleic acid—were prepared as a renewable raw material. Short (I), medium (II), and long (III) alkyd resins were synthesized using oil, glycerol, and phthalic anhydride (PA) in different proportions. Prunus mahaleb seed oil (PMSO), which contains conjugated linolenic acid, was blended with CCSO in different proportions to examine the effect of PMSO on the film properties of alkyd resins (IV and V). All synthesized resins were characterized by FTIR and 1H NMR spectroscopic analysis. These More >

  • Open Access

    ARTICLE

    Enzymatic Synthesis and Characterization of Biodegradable Poly(w-pentadecalactone-co-e-caprolactone) Copolymers

    Cansu Ulker*, Yuksel Guvenilir

    Journal of Renewable Materials, Vol.6, No.6, pp. 591-598, 2018, DOI:10.7569/JRM.2017.634189

    Abstract As an alternative biodegradable aliphatic polyester, poly(w-pentadecalactone-co-ε-caprolactone) copolymer was synthesized via enzymatic ring-opening polymerization. A new biocatalyst, Candida antarctica lipase B, immobilized onto rice husk ash was used for catalysis. Reactions were carried out at various temperatures and periods for varied copolymer compositions in order to obtain the highest molecular weight copolymer. The best reaction parameters were found to be 80 °C and 6 hours and molecular weights increased proportionally with the amount of w-pentadecalactone (w-PDL). The molecular structure of copolymer with 75% weight ratio of w-PDL (Mn = 19720 g/mol) was characterized by proton More >

  • Open Access

    ARTICLE

    Preparation and Characterization of Eco-friendly Carboxymethyl Cellulose Antimicrobial Nanocomposite Hydrogels

    Sawsan Dacrory1*, Hussein Abou-Yousef1, Ragab E. Abou-Zeid1, Samir Kamel1, Mohamed S. Abdel-Aziz2, Mohamed Elbadry3

    Journal of Renewable Materials, Vol.6, No.5, pp. 536-547, 2018, DOI:10.7569/JRM.2017.634190

    Abstract Carboxymethyl cellulose hydrogels were developed through crosslinking process using eco-friendly crosslinkers such as maleic, succinic, and citric acids. Carboxymethyl cellulose was prepared from the cellulosic fraction of olive industry residues. A series of hydrogels with varying crosslinker acid concentrations, reaction times, and reaction temperatures was produced to study the swelling capacities and gel fraction of the obtained hydrogels. Additional study pertains to the preparation of antimicrobial nanocomposite hydrogels through in-situ incorporation of the silver nanoparticles during the crosslinking reaction. Silver nanoparticles were prepared by reduction of AgNO3with leaves of Ricinus communis. The particle size of More >

  • Open Access

    ARTICLE

    Isolation and Characterization of Nanocellulose Obtained from Industrial Crop Waste Resources by Using Mild Acid Hydrolysis

    Galia Moreno, Karla Ramirez, Marianelly Esquivel, Guillermo Jimenez*

    Journal of Renewable Materials, Vol.6, No.4, pp. 362-369, 2018, DOI:10.7569/JRM.2017.634167

    Abstract Cellulose, microcrystalline cellulose and nanocellulose were prepared from three agricultural waste resources: pineapple leaf (PALF), banana rachis (BR), and sugarcane bagasse (SCB). Each waste resource was first converted into microcrystalline cellulose which was subsequently converted into cellulose nanoparticles by using mild (30% w/v) and strong (60% w/v) sulfuric acid concentrations for extraction. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA) were used to characterize each waste resource and extracted cellulosic materials. Furthermore, nanocelluloses were studied by zeta potential, size analysis, and transmission electron microscopy (TEM). Cellulose nanowhiskers were successfully obtained and More >

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