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  • Open Access

    ARTICLE

    Structural and ultrastructural characterization of zebu (Bos indicus) spermatozoa

    MARIA CAROLINA AQUINO LUQUE, SÔNIA NAIR BÁO

    BIOCELL, Vol.30, No.1, pp. 33-38, 2006, DOI:10.32604/biocell.2006.30.033

    Abstract The ultrastructure of normal, ejaculated spermatozoa of Bos indicus was studied by means of electron microscopy, being evaluated in two principal parts, the head and the tail. The head is flat, oval or paddle-shaped with a square base, which provides a concave recess for the insertion of the tail. The acrosome tightly covers the anterior two thirds of the nucleus. A distinct unilateral acrosomal bulge was observed along the apical edge of the head. The equatorial region demarcates the acrosome from the post-equatorial region that covers the caudal one third of the nucleus. The classical 9+9+2 More >

  • Open Access

    ARTICLE

    Genomic cloning and characterization of a PPA gene encoding a mannose-binding lectin from Pinellia pedatisecta

    JUAN LIN1, XUANWEI ZHOU2, JIONG FEI2, ZHIHUA LIAO1, WANG JIN1, XIAOFEN SUN1,*, KEXUAN TANG1,2,*

    BIOCELL, Vol.30, No.1, pp. 15-25, 2006, DOI:10.32604/biocell.2006.30.015

    Abstract A gene encoding a mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was isolated from leaves of Pinellia pedatisecta using genomic walker technology. The ppa contained an 1140-bp 5’-upstream region, a 771-bp open reading frame (ORF) and an 829-bp 3’-downstream region. The ORF encoded a precursor polypeptide of 256 amino acid residues with a 24-amino acid signal peptide. There were one putative TATA box and six possible CAAT boxes lying in the 5’-upstream region of ppa. The ppa showed significant similarity at the nucleic acid level with genes encoding mannose-binding lectins from other Araceae species such as Pinellia ternata, Arisaema heterophyllum, Colocasia esculenta More >

  • Open Access

    ARTICLE

    Molecular cloning and characterization of a novel mannosebinding lectin cDNA from Zantedeschia aethiopica

    ZHONGHAI CHEN1, YONGZHEN PANG1, XIAOJUN LIU1, XINGLONG WANG1, ZHONGXIANG DENG1, XIAOFEN SUN1, KEXUAN TANG1,2

    BIOCELL, Vol.29, No.2, pp. 187-193, 2005, DOI:10.32604/biocell.2005.29.187

    Abstract Using RNA extracted from Zantedeschia aethiopica young leaves and primers designed according to the conservative regions of Araceae lectins, the full-length cDNA of Z. aethiopica agglutinin (ZAA) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of zaa was 871 bp and contained a 417 bp open reading frame (ORF) encoding a lectin precursor of 138 amino acids. Through comparative analysis of zaa gene and its deduced amino acid sequence with those of other Araceae species, it was found that zaa encoded a precursor lectin with signal peptide. Secondary and three-dimensional structure analyses showed that ZAA More >

  • Open Access

    ARTICLE

    Computational Characterization and Evaluation of Deformation Behavior of Spherulite of High Density Polyethylene in Mesoscale Domain

    Y. Tomita 1, M. Uchida 1

    CMES-Computer Modeling in Engineering & Sciences, Vol.10, No.3, pp. 239-248, 2005, DOI:10.3970/cmes.2005.010.239

    Abstract In this study, we clarified the micro- to mesoscopic deformation behavior of a semicrystalline polymer by employing a large-deformation finite element homogenization method. The crystalline plasticity theory with a penalty method for the inextensibility of the chain direction and the nonaffine molecular chain network theory were applied for the representation of the deformation behavior of the crystalline and amorphous phases, respectively, in the composite microstructure of the semicrystalline polymer. The 3D structure of lamellae in the spherulite of high-density polyethylene was modeled, and the tensile and compressive deformation behaviors were investigated. A series of computational More >

  • Open Access

    ARTICLE

    Transient Response in Cross-Ply Laminated Cylinders and Its Application to Reconstruction of Elastic Constants

    X. Han1,2,3, G. R. Liu1,2, G. Y. Li 1

    CMC-Computers, Materials & Continua, Vol.1, No.1, pp. 39-50, 2004, DOI:10.3970/cmc.2004.001.039

    Abstract An efficient hybrid numerical method is presented for investigating transient response of cross-ply laminated axisymmetric cylinders subjected to an impact load. In this hybrid numerical method, the laminated cylinder is divided into layered cylindrical elements in the thickness direction. The Hamilton principle is used to develop governing equations of the structure. The displacement response is determined by employing the Fourier transformations and the modal analysis. Numerical examples for analyzing transient waves have been provided in axisymmetric laminated cylindrical structures, both for thin cylindrical shells and thick cylinders.
    A computational inverse technique is also presented for More >

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