Xingxiang Liu^*†, Lin Cui^†, Dong Hua^*‡

Oncology Research, Vol.27, No.1, pp. 99-106, 2019, DOI:10.3727/096504018X15195193936573

Abstract We aimed to investigate the significant role of long noncoding RNA X inactive specific transcript (XIST) in regulating tumor metastasis in colorectal cancer (CRC), as well as its possible mechanism. Expression of lncRNA XIST in CRC tissues and CRC cells was detected. CRC cells were transfected with pc-XIST, blank control si-XIST, or si-control, and then the effects of lncRNA XIST on CRC cell migration and invasion were investigated, along with the interaction between lncRNA XIST and miR-137. lncRNA XIST was upregulated in CRC tissues. Compared with HT29 cells that had low metastatic potential, XIST was… More >

Hai Liu^*, Xuanxuan Wang^*, Aihua Huang^†, Huaping Gao^‡, Yikan Sun^§, Tingting Jiang^*, Liming Shi^*, Xianjie Wu^¶, Qinghua Dong^#, Xiaonan Sun^*

Oncology Research, Vol.27, No.1, pp. 29-38, 2019, DOI:10.3727/096504018X15179694020751

Abstract Artemis is a key protein of NHEJ (nonhomologous end joining), which is the major pathway for the repair of IR-induced DSBs in mammalian cells. However, the expression of Artemis in tumors and the influence of silencing Artemis on tumor sensitivity to radiation have not been investigated fully. In this study, we investigated how the expression levels of Artemis may affect the treatment outcome of radiotherapy and chemotherapy in colorectal cancer cells. First, we found that the expression of Artemis is strong in some human rectal cancer samples, being higher than in adjacent normal tissues using… More >

Fan-Chun Meng, Jun-Kai Lin

Oncology Research, Vol.27, No.2, pp. 139-146, 2019, DOI:10.3727/096504018X15185747911701

Abstract Inhibition of tumor metastasis is one of the most important purposes in colorectal cancer (CRC) treatment. This study aimed to explore the effects of liquiritigenin, a flavonoid extracted from the roots of Glycyrrhiza uralensis Fisch, on HCT116 cell proliferation, invasion, and epithelial-to-mesenchymal transition (EMT). We found that liquiritigenin significantly inhibited HCT116 cell proliferation, invasion, and the EMT process, but had no influence on cell apoptosis. Moreover, liquiritigenin remarkably reduced the expression of runt-related transcription factor 2 (Runx2) in HCT116 cells. Overexpression of Runx2 obviously reversed the liquiritigenininduced invasion and EMT inhibition. Furthermore, liquiritigenin inactivated the More >

Qiuying Quan^*1, Fengyun Zhong^†1, Xinwei Wang^‡, Kai Chen^§, Lingchuan Guo^*

Oncology Research, Vol.27, No.7, pp. 779-788, 2019, DOI:10.3727/096504018X15442985680348

Abstract The aim of this study was to investigate the underlying mechanisms that transforming growth factor- (TGF- )-mediated epithelial-to-mesenchymal transition (EMT) in tumor cells contributes to 5-FU resistance. A series of experiments involving cell viability and caspase activity analyses, siRNA transfection, RNA isolation, and quantitative-PCR (qPCR) assay, cell migration analysis, Western blotting analysis of total protein and membrane protein were performed in this study. Mouse xenograft model was used to determine the effect of the PAR2 inhibitor in vivo. In this study, we found that protease-activated receptor 2 (PAR2) induction in 5-FU therapy is correlated with… More >

Hua Yunqi^*1, Yin Fangrui^†1, Yang Yongyan^*, Jin Yunjian^*, Zhang Wenhui^*, Cao Kun^*, Li Min^*, Liu Xianfeng^*, Ba Caixia^*

Oncology Research, Vol.27, No.3, pp. 311-316, 2019, DOI:10.3727/096504018X15220579006875

Abstract Emerging evidence indicates that microRNAs (miRNAs) are often aberrantly expressed in human cancers. Meanwhile, the importance of miRNAs in regulating multiple cellular biological processes has been appreciated. The aim of this study was to investigate the significance of miR-455 and identify its possible mechanism in regulating colorectal cancer (CRC) progression. We found that the expression of miR-455 was sharply reduced in CRC tissues and cell lines. Importantly, the low expression of miR-455 was associated with poor overall survival of CRC patients. Overexpression of miR-455 in CRC cell lines significantly inhibited cell proliferation and migration in More >

Chijiang Gu^*, Mingyuan Zhang^*, Weiliang Sun^*, Changzheng Dong^†

Oncology Research, Vol.27, No.5, pp. 515-524, 2019, DOI:10.3727/096504018X15166183598572

Abstract Colorectal cancer (CRC) is a common clinical cancer that remains incurable in most cases. miRNAs are reported to play a part in the development of various tumors. In the present study, we found that miR-324-5p was downregulated in CRC cells, while ELAV (embryonic lethal, abnormal vision, Drosophila)-like protein 1 (ELAVL1) showed a higher expression. miR-324-5p transfection significantly inhibited the proliferation as well as invasion in both SW620 and SW480 cells. miR-324-5p mimic transfection markedly decreased the expression of ELAVL1. Luciferase reporter gene assay confirmed that ELAVL1 is a direct target of miR- 324-5p. Furthermore, cancer invasion More >

S. Deivalakshmi^*, P. Palanisamy¹, X. Z. Gao²

Intelligent Automation & Soft Computing, Vol.25, No.3, pp. 459-471, 2019, DOI:10.31209/2018.100000001

Abstract The main aim of this paper is to propose a new technique for enhancing the contrast of dark images using Dynamic Stochastic Resonance (DSR) and Multi Wavelet Transform (MWT), which is computationally more efficient than the conventional methods. In the work, for enhancing the contrast of dark images, the intrinsic noise (darkness) of dark images has been used. The proposed MWT-based DSR scheme (MWT-DSR) can yield better performances in terms of visual information and color preservation than already reported techniques. The desired output response is validated by the Relative Contrast Enhancement Factor (F), Perceptual Quality More >

Marina Tsvetova¹, Lev Elkonin^1,*, Yulia Italianskaya¹

Phyton-International Journal of Experimental Botany, Vol.88, No.4, pp. 389-401, 2019, DOI:10.32604/phyton.2019.07485

Abstract Apomictic seed development is a complex process including formation of unreduced embryo sac, parthenogenetic embryo development from the egg cell, and endosperm formation either autonomously, or due to fertilization of polar nuclei by the sperm (under pseudogamous form of apomixis). In the latter case, an obstacle to the normal endosperm development is disturbance of maternal (m) -to-paternal (p) genomic ratio 2m: 1p that occurs in the cases of pollination of unreduced embryo sac with haploid sperms. Usage of tetraploid pollinators can overcome this problem because in such crosses maternal-to-paternal genomic ratio is 4m: 2p that… More >

Xiaoning Qin¹, Liqing Yuan², Hongxun Ruan¹, Lin Lin¹

BIOCELL, Vol.43, No.2, pp. 89-98, 2019, DOI:10.32604/biocell.2019.06352

Abstract Interleukin-22 (IL-22) is a member of IL-10 cytokine family which is expressed in activated T cells predominantly and in activated natural killer cells at lower levels. Previous studies have demonstrated the link between elevated levels of IL-22 and disease severity of psoriasis, Crohn’s disease, rheumatoid arthritis and interstitial lung diseases. However, the function of IL-22 in the development and progression of colorectal cancer (CRC) remains elusive. In this study, we first evaluated the IL-22/IL-22R1 level in CRC patients, and found that tumor tissues have more active expression of IL-22 and IL-22R1 than normal tissues,… More >

Hala Abdulaziz M ALWARTHAN¹, Mohammad Saud AL ANAZI¹, Narasimha R. PARINE¹, Ramesa Shafi BHAT^1,*, Ghadah ALAMRO¹, Ftoon ALJARBOU¹, Sooad K AL-DAIHAN¹

BIOCELL, Vol.43, No.2, pp. 51-58, 2019, DOI:10.32604/biocell.2019.07019

Abstract Tankyrase1 plays an essential role in cancer progression by regulating telomere length. The study aimed to determine expression of TNKS1 and its regulation in colorectal cancer (CRC) in 20 samples from Saudi patients. mRNA expression of TNKS1 in CRC and paired normal tissues was measured by qRT-PCR. Epigenetic modification of TNKS1 promoter was determined by methylation-specific PCR while somatic mutation was analyzed by Sanger sequencing in exon 10 of the gene. All cancerous and normal tissues expressed TNKS1, but level of expression in CRC tissues was significantly associated with tumor stage though no other parameters; age, gender,… More >